IFO50276 A2058

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50276 Cell Name   A2058
Profile   malignant melanoma, metastasis to lymph node Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   43
Identity   available Tissue for Primary Cancer   melanoma
Case history   malignant melanoma Metastasis   Yes
Tissue Metastasized   lymph node Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   malignant melanoma, metastasis to lymph node
Classify   tumor Established by   Fabricant, R. N. et al.
Registered by   Kanamaru,T. Regulation for Distribution   
Comment    Year   1990
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   melanoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1059
Reference
Pubmed id:3003087Laminin increases the release of type IV collagenase from malignant cells.
Turpeenniemi-Hujanen T,Thorgeirsson UP,Rao CN,Liotta LA
J Biol Chem. 1986 Feb 5;261(4):1883-9
Pubmed id:6933558Carbohydrate requirement for expression and stability of acetylcholine receptor on the surface of embryonic muscle cells in culture.
Prives JM,Olden K
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5263-7
Pubmed id:286311Induction of terminal differentiation in human promyelocytic leukemia cells by tumor-promoting agents.
Huberman E,Callaham MF
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1293-7
Pubmed id:265522Nerve growth factor receptors on human melanoma cells in culture.
Fabricant RN,De Larco JE,Todaro GJ
Proc Natl Acad Sci U S A. 1977 Feb;74(2):565-9

Images



LOT Information

Cell No.   IFO50276 Cell Name   
LOT No.   01282005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 30 hrs Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P17 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50276 Cell Name   A2058
LOT No.   1070 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free (previously treated with MC210, BM1, BM2)
Passage Number   Unknown (13 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50276 Cell Name   A2058
LOT No.   12252014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Ampoule was mislabeled as A2059. Correctly, A2058. Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (18 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,12
D13S317:13,14
D7S820:11
D16S539:9,13
VWA:14,18
TH01:7,9
AM:X,Y
TPOX:8
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   Ampoule was mislabeled as A2059. Correctly, A2058.
Cell No.   IFO50276 Cell Name   A2058
LOT No.   10202016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.4 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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