IFO50289 Becker
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50289 | Cell Name | Becker |
|---|---|---|---|
| Profile | astrocytoma, GFAP-negative | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 57 |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | astrocytoma, grade III-IV | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | astrocytoma, GFAP-negative |
| Classify | tumor | Established by | Pfreundschuh, M. et al. |
| Registered by | Kaji,K. | Regulation for Distribution | |
| Comment | Year | 1990 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:11542786 | Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams. Suzuki M,Kase Y,Kanai T,Ando K Adv Space Res. 1998;22(4):561-8 |
| Pubmed id:283420 | Serological analysis of cell surface antigens of malignant human brain tumors. Pfreundschuh M,Shiku H,Takahashi T,Ueda R,Ransohoff J,Oettgen HF,Old LJ Proc Natl Acad Sci U S A. 1978 Oct;75(10):5122-6 |
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LOT Information
| Cell No. | IFO50289 | Cell Name | Becker |
|---|---|---|---|
| LOT No. | 988 | Lot Specification | distribution |
| Medium | E.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1 x 10^6 | |
| Viability at cell freezing (%) | 85 | Antibiotics Used | free |
| Passage Number | Unknown (>4 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:9 D7S820:11 D16S539:10,14 VWA:16,17 TH01:8,9 AM:X,Y TPOX:8,11 CSF1PO:12,13 |
|
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information | Lot# is labeled as R597F988. Cell# isn't labeled. |
| Cell No. | IFO50289 | Cell Name | Becker |
|---|---|---|---|
| LOT No. | 02022017 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 0.5 - 1.7 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | 23 - 42 hrs. | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 94.2 | Antibiotics Used | free |
| Passage Number | Unknown (5 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94.2 | Additional information |