IFO50294 MEG-01SSF

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50294 Cell Name   MEG-01SSF
Profile   leukemia, chronic megakaryoblastic, MEG-01s-derivative adapted to serum-free medium Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   56
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   chronic leukemia Metastasis   
Tissue Metastasized    Genetics   serum-free adapted subline of MEG-01s (IFO 50473)
Life Span   infinite Crisis PDL   
Morphology   round Character   leukemia, chronic megakaryoblastic, MEG-01s-derivative adapted to serum-free medium
Classify   variant Established by   Takeuchi, M.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1990
Medium   G5 medium (D.MEM (high glucose) with 5ug/ml insulin, 50ug/ml transferrin, 10nM hydrocortisone, 10ng/ml biotin, 30nM Na-selenite, 5ng/ml bFGF, 10ng/ml EGF) Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3023
Reference
Pubmed id:1367286Properties of a cell line (MEG-01SSF) of human megakaryoblastic leukemia cells.
Takeuchi M,Satoh M,Ogura M,Saito H,Takeuchi K
Res Commun Inst Ferment. 1991;(15):22-9

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LOT Information

Cell No.   IFO50294 Cell Name   MEG-01SSF
LOT No.   772 Lot Specification   distribution
Medium   G5 medium Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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