IFO50359 KNS-81

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50359 Cell Name   KNS-81
Profile   glioma, GFAP and S-100-positive, NSE-negative Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   65
Identity   available Tissue for Primary Cancer   neural
Case history   glioma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibrobrast-like Character   glioma, GFAP and S-100-positive, NSE-negative
Classify   tumor Established by   Takeshita, I.
Registered by   Takeshita,I. Regulation for Distribution   
Comment    Year   1991
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
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LOT Information

Cell No.   IFO50359 Cell Name   KNS-81
LOT No.   1019 Lot Specification   distribution
Medium   D.MEM, 95% ; FBS, 5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability (%)   92 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Additional information   The vials are labeled as KNS-81 R801F1019.
Cell No.   IFO50359 Cell Name   KNS-81
LOT No.   08052014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   3.6 - 7.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 45 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability (%)   74.1 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,12
D13S317:9
D7S820:11
D16S539:10,12
VWA:17,18
TH01:7,9
AM:X,Y
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
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