IFO50359 KNS-81

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50359 Cell Name   KNS-81
profile   glioma, GFAP and S-100-positive, NSE-negative Alias   
animal   human strain   
genus   Homo species   sapiens
sex   M age   65
identity   available tissue   neural
case history   glioma metastasis   
metastorgn    genetics   
lifespan   infinite crisispdl   
morphology   fibrobrast-like charact   glioma, GFAP and S-100-positive, NSE-negative
classify   tumor originator   Takeshita, I.
register   Takeshita,I. regulation   
comment    dyear   1991
medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum pasmethod   trypsin-EDTA
pascellno    race   
co2conc   5 % tohroku   registered
preporgn   brain vrsummary   tested
vrsdetect   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] tissueform   
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LOT Information

Cell No.   IFO50359 Cell Name   KNS-81
LOT No.   1019 LOTSPEC   distribution
MEDIUM   D.MEM, 95% ; FBS, 5% GROWTEMP   37 C
PASCELLNO    PASMETHOD   trypsin-EDTA
GROWTHRATE    FREEZCONC   1 x 10^6
VIABLFREEZ   92 ANTIBIOTIC   free
PASSAGE   Unknown (6 at IFO) PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   NP, G6PD(type B), MD
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD    EXOGENE   
FMEDIUM   10% DMSO, 20% serum (final conc.) - culture medium CO2   
RFLP    Additional information   The vials are labeled as KNS-81 R801F1019.
Cell No.   IFO50359 Cell Name   KNS-81
LOT No.   08052014 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10091) GROWTEMP   37 C
PASCELLNO   3.6 - 7.0 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
GROWTHRATE   approx. 45 hrs FREEZCONC   2.0 x 10^6
VIABLFREEZ   74.1 ANTIBIOTIC   free
PASSAGE   Unknown (6 at bank) PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   D5S818:9,12
D13S317:9
D7S820:11
D16S539:10,12
VWA:17,18
TH01:7,9
AM:X,Y
TPOX:8,11
CSF1PO:9,12
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    Additional information   
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