IFO50373 TKC2
Cell information
Important Notice(s)Growth temperature of this cell line is 33 degree centigrade !!
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50373 | Cell Name | TKC2 |
|---|---|---|---|
| Profile | cAMP synthesis is stimulated by arginine vasopressin in the cells. Growth temperature is 33 C. | Other Name | |
| Animal | mouse | Strain | C57BL/6 transgenic |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | tuble cell | Metastasis | |
| Tissue Metastasized | Genetics | TG mouse harboring tsSV40 large T antigen gene | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | tubule cell, tsSV40 large T-immortalized (ts mutant) | |
| Classify | mutant | Established by | Yanai, N. et al. |
| Registered by | Yanai,N. | Regulation for Distribution | |
| Comment | Year | 1992 | |
| Medium | RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ul/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin. Ts cell line (33 C). | Methods for Passages | 25 ug/ml pronase-Hanks'BSS |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1663919 | A tubule cell line established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Yanai N,Satoh T,Kyo S,Abe K,Suzuki M,Obinata M Jpn J Cancer Res. 1991 Dec;82(12):1344-8 |
LOT Information
| Cell No. | IFO50373 | Cell Name | TKC2 |
|---|---|---|---|
| LOT No. | 04192002 | Lot Specification | distribution |
| Medium | RITC80-7 medium with 10 ng/ml EGF, 10 ug/ml transferrin, 1 ug/ml insulin, 5 ul/L monoethanolamine and 2% FBS | Temperature | 33 C !! |
| Cell Density at Seeding | 1.0 x 10^5 cells/ml | Methods for Passages | 25 ug/ml pronase in Hanks' BSS |
| Doubling Time | D.T. = ca 27 hrs | Cell Number in Vial (cells/1ml) | 2.3 x 10^6 |
| Viability at cell freezing (%) | 82.9 | Antibiotics Used | free |
| Passage Number | P15 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50373 | Cell Name | TKC2 |
|---|---|---|---|
| LOT No. | 874 | Lot Specification | seed/distribution |
| Medium | RITC80-7 with 2%FCS, 10ng/ml EGF, 5 lg/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin. | Temperature | 33 C |
| Cell Density at Seeding | Methods for Passages | 25 ug/ml pronase-Hanks'BSS | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | free, pretreatment with MC210 | |
| Passage Number | >8 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, PepB, LD |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Exoteric Gene | tsSV40 large T antigen gene | |
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |