IFO50377 FLS3

Cell information

Important Notice(s)

RITC80-7 medium

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50377 Cell Name   FLS3
Profile   This cell line supports B-cell differentiation. (Blood, 77,2612,(1991)). Other Name   
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   liver
Case history   liver stromal cell Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   liver stromal cell line
Classify    Established by   Ohneda, O. et al.
Registered by   Yanai,N. Regulation for Distribution   
Comment    Year   1992
Medium   RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ul/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin Methods for Passages   25 ug/ml pronase-Hanks'BSS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5503
Reference
Pubmed id:2133543Microenvironment created by stromal cells is essential for a rapid expansion of erythroid cells in mouse fetal liver.
Ohneda O,Yanai N,Obinata M
Development. 1990 Oct;110(2):379-84




LOT Information

Cell No.   IFO50377 Cell Name   FLS3
LOT No.   1025 Lot Specification   distribution
Medium   RITC80-7 with 2%FCS, 10ng/ml EGF, 5 ul/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin Temperature   37 C
Cell Density at Seeding    Methods for Passages   25 ug/ml pronase-Hanks'BSS
Doubling Time    Cell Number in Vial (cells/1ml)   9 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   >21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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