IFO50380 TLR3

Cell information

Important Notice(s)

RITC80-7 medium

Growth temperature of this cell line is 33 degree centigrade !!

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50380 Cell Name   TLR3
Profile   P450IA1 mRNA is induced by methylcholanthrene. Growth temperature is 33 C. Other Name   
Animal   mouse Strain   C57BL/6 transgenic
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history   hepatocyte Metastasis   
Tissue Metastasized    Genetics   TG mouse harboring tsSV40 large T antigen gene
Life Span   infinite Crisis PDL   
Morphology    Character   hepatocyte immortalized by the tsSV40 large T antigen gene. Permissive temperature is 33 C.
Classify   transformed Established by   Yanai, N. et al.
Registered by   Yanai,N. Regulation for Distribution   
Comment    Year   1992
Medium   RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ul/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin Methods for Passages   25 ug/ml pronase-Hanks'BSS, collagen-coated substratum is required
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5601
Reference
Pubmed id:1655502Hepatocyte cell lines established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
Yanai N,Suzuki M,Obinata M
Exp Cell Res. 1991 Nov;197(1):50-6




LOT Information

Cell No.   IFO50380 Cell Name   TLR3
LOT No.   06072002 Lot Specification   distribution
Medium   RITC80-7 with 2% FCS, 10ng/ml EGF, 5ul/L monoethanolamine, 10ug/ml transferrin, 1ug/ml insulin Temperature   33 C. This cell line does not grow at 37 C.
Cell Density at Seeding   1.5-2.0 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Collagen-coat is needed.
Doubling Time    Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   91.2 Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP. MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50380 Cell Name   TLR3
LOT No.   859 Lot Specification   seed/distribution
Medium   RITC80-7 with 2%FCS, 10ng/ml EGF, 5 ul/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin Temperature   33 C
Cell Density at Seeding    Methods for Passages   25 ug/ml pronase-Hanks'BSS ; collagen-coated culture dish
Doubling Time    Cell Number in Vial (cells/1ml)   4 x 10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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