IFO50409 NCTC clone 929

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50409 Cell Name   NCTC clone 929
Profile   A clone of L cells, and often referred as L929. The L cells was established from normal subcutaneous areolar and adipose tissue. Other Name   
Animal   mouse Strain   C3H/An
Genus   Mus Species   musculus
Sex   M Age   
Identity    Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast, subclone of strain L
Classify    Established by   Sanford, K. K.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1993
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   connective tissue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0462
Reference
Pubmed id:178611Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
Sundström C,Nilsson K
Int J Cancer. 1976 May 15;17(5):565-77
Pubmed id:13655076Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).
WAYMOUTH C
J Natl Cancer Inst. 1959 May;22(5):1003-17
Pubmed id:13271674The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure.
EAGLE H
J Exp Med. 1955 Nov 1;102(5):595-600
Pubmed id:13035407A quantitative study of the effect of certain chemically defined media on the proliferation in vitro of strain L cells from the mouse.
EVANS VJ,SHANNON JE Jr,BRYANT JC,WALTZ HK,EARLE WR,SANFORD KK
J Natl Cancer Inst. 1953 Feb;13(4):773-84




LOT Information

Cell No.   IFO50409 Cell Name   
LOT No.   04262010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids (0.1 mM each) and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 4 - 5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 - 25 hrs Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50409 Cell Name   
LOT No.   04042008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   P11* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50409 Cell Name   NCTC clone 929
LOT No.   1075 Lot Specification   distribution
Medium   E.MEM with NEAA, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin--EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   10 x 10^5
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50409 Cell Name   NCTC clone 929
LOT No.   08042016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Cell name of ampoule label is incorrect. Correctly NCTC clone 929. Temperature   37 C
Cell Density at Seeding   4.2 - 6.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 23 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   88 Additional information   Mislabeled as NTCT clone 929 on vials. Correct name is NCTC clone 929.
Cell No.   IFO50409 Cell Name   NCTC clone 929
LOT No.   10062023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   2.7 - 3.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
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