-Detailed Information [IFO50416]-

-------- 培地 ---------

本細胞の培養には、基礎培地としてDMEM (Dulbecco's modified Eagle's Medium)を指定しています。解凍、培養・維持に際しては用いるDMEMの処方は次の点に注意してください。

(1) 必ず低グルコース(1 g/L)のDMEMを用いてください。

低グルコースのDMEMが標準のDMEMの処方です。高グルコースのDMEMは変法です。解凍・培養に際して高グルコースのDMEMを用いると、培地馴化の問題で生存不良・増殖不良を招く恐れがあります。

(2) グルタミンとピルビン酸ナトリウムが含有されていることをご確認ください。

グルタミンは必須アミノ酸であり、不含の培地を用いると細胞が死滅します。

---- 解凍時の注意 -----

3T3-L1細胞では、解凍からの状態の回復が細胞密度にかなり依存します。解凍時に細胞密度が低いと、生存不良・増殖不良を招く恐れがあります

このため、解凍時には、1バイアルあたりの全細胞を6 cmシャーレ1枚、あるいは25 cm2フラスコ1本に播種するようにしてください。培地量は5 mLが適切です。

解凍時には凍害保護剤を除くための遠心洗浄を必ず行ってください。


[Media]

1. Do not use high-glucose DMEM, it may cause proliferation disorder. Low-glucose DMEM should be used to culture.

2. Make sure the media includes Glutamine and sodium pyruvate.

[Note for thawing]

1. The appropriate culture volume would be 5 ml per vial in a 25cm2 flask or a 6cm dish. Excessive dilution may lead to low viability.

2. Centrifugal separation for removing frost damage-protecting agent is indispensable.  



[Close]

JCRB No.	IFO50416	Cell Name	3T3-L1
Profile	3T3 L1 cell line is a substrain of 3T3-Swiss albino cell line. 3T3 L1 cells convert adipose-like cells at confluent.	Other Name
Animal	mouse	Strain	Swiss albino
Genus	Mus	Species	musclus
Sex		Age
Identity		Tissue for Primary Cancer	embryo
Case history		Metastasis
Tissue Metastasized		Genetics	a substrain of 3T3-Swiss albino cell line
Life Span	infinite	Crisis PDL
Morphology	fibroblast-like	Character	fibroblast, 3T3-Swiss derivative, adipocyte differentiation
Classify	normal	Established by	Green, H. et al.
Registered by	Takeuchi,M.	Regulation for Distribution
Comment		Year	1993
Medium	Dulbecco's modified Eagle's medium with 10% calf serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	embryo
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	not tested	Detected RNA Virus	not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0123

07232012
07032008
12142004
07122001
1085
02202015
08012016
02212018
04192023

Cell No.	IFO50416	Cell Name
LOT No.	07232012	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine.	Temperature	37 C
Cell Density at Seeding	1.8 - 5.6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 28 hrs	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	92.2	Antibiotics Used	free
Passage Number	Unknown (10 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% CS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50416	Cell Name
LOT No.	07032008	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% calf serum	Temperature	37 C
Cell Density at Seeding	2 - 5 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	31 hrs	Cell Number in Vial (cells/1ml)	9.3 x 10^5
Viability at cell freezing (%)	96.4	Antibiotics Used	free
Passage Number	Unknown (9 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% CS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50416	Cell Name
LOT No.	12142004	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% calf serum	Temperature	37 C
Cell Density at Seeding	2-4 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	ca. 2.2 days	Cell Number in Vial (cells/1ml)	2.6 x 10^5
Viability at cell freezing (%)	94.2	Antibiotics Used	free
Passage Number	P10*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as mouse by NP, MD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% CS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50416	Cell Name
LOT No.	07122001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% calf serum	Temperature	37 C
Cell Density at Seeding	1-2 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in PBS(-)
Doubling Time		Cell Number in Vial (cells/1ml)	2.0 x 10^5
Viability at cell freezing (%)	92.2	Antibiotics Used	free
Passage Number	P10*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50416	Cell Name	3T3-L1
LOT No.	1085	Lot Specification	distribution
Medium	D.MEM, 90% ; calf serum, 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	4 x 10^5
Viability at cell freezing (%)	85	Antibiotics Used	free
Passage Number	Unknown (6 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD, MD, AST
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50416	Cell Name	3T3-L1
LOT No.	02202015	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine.	Temperature	37 C
Cell Density at Seeding	2.7 - 4.3 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to become full confluency.
Doubling Time	D.T. = approx. 54 hrs	Cell Number in Vial (cells/1ml)	8.7 x 10^5
Viability at cell freezing (%)	66.4	Antibiotics Used	free
Passage Number	Unknown (11 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% newborn calf serum - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	66.4	Additional information

Cell No.	IFO50416	Cell Name	3T3-L1
LOT No.	08012016	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine.	Temperature	37 C
Cell Density at Seeding	approx. 2 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to become full confluency.
Doubling Time	approx. 39 hrs	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	80.0	Antibiotics Used	free
Passage Number	Unknown (10 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% NCS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	80.0	Additional information	labeled as 3T3-1 on vials.

Cell No.	IFO50416	Cell Name	3T3-L1
LOT No.	02212018	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010). DMEM is 1 g/L glucose (low glucose) type and must contain L-glutamine.	Temperature	37 C
Cell Density at Seeding	3 - 5 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to become full confluency.
Doubling Time	approx. 28 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	95.3	Antibiotics Used	free
Passage Number	Unknown (10 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	95.3	Additional information

Cell No.	IFO50416	Cell Name	3T3-L1
LOT No.	04192023	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% newborn calf serum (NCS; Biowest S0750, lot S14368S0750)	Temperature	37 C
Cell Density at Seeding	2.2 - 2.8 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to become full confluency.
Doubling Time	approx. 39 hrs.	Cell Number in Vial (cells/1ml)	7.4 x 10^5
Viability at cell freezing (%)	97	Antibiotics Used	free
Passage Number	Unknown (10 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% NCS- DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	96	Additional information

JCRB Cell Bank

IFO50416 3T3-L1

Cell information

LOT Information