IFO50425 C6-SF2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50425 Cell Name   C6-SF2
Profile   C6 subline adapted to serum-free medium Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   glial tumor Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round with processes Character   C6 subline adapted to serum-free medium
Classify   variant Established by   Yoshida, T.
Registered by   Yoshida, T. Regulation for Distribution   
Comment    Year   1993
Medium   D.MEM(high glucose) with 5ug/ml insulin, 50ug/ml transferrin, 10ng/ml biotin and 30nM Na-selenite. Methods for Passages   0.1% crystalline trypsin-0.1% soybean trypsin inhibitor (Sigma type I-S)
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6336
Reference
Pubmed id:4873531Differentiated rat glial cell strain in tissue culture.
Benda P,Lightbody J,Sato G,Levine L,Sweet W
Science. 1968 Jul 26;161(3839):370-1




LOT Information

Cell No.   IFO50425 Cell Name   C6-SF2
LOT No.   806 Lot Specification   seed/distribution
Medium   D.MEM(high glucose) with 5 ug/ml insulin, 50 ug/mlTf, 10ng/ml biotin, 30nM Na-selenite Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.1% crystalline trypsin-soybean trypsin inhibitor
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   62 Antibiotics Used   free
Passage Number   P13 after serum-free PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50425 Cell Name   C6-SF2
LOT No.   02232016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose type) with 5 ug/ml insulin, 50 ug/ml transferrin, 10 ng/ml biotin and 30 nM Na-selenite. (serum-free) Temperature   37 C
Cell Density at Seeding   1.1 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were dissociated with 0.1% crystalline trypsin in PBS (-). The trypsin activity should be terminated by 0.1% soybean trypsin inhibitor (Sigma type I-S). Cells grow with forming cell aggregates, and loosely attach to substratum.
Doubling Time   approx. 50 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   81 Antibiotics Used   free
Passage Number   P18 (after serum-free culture) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   81 Additional information   
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