IFO50428 CMK-86
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50428 | Cell Name | CMK-86 |
|---|---|---|---|
| Profile | This cell line expresses GPIIb/IIIa. Contains alpha-granule. Contains platelet peroxidase activity. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | acute megakaryoblastic leukemia | Metastasis | |
| Tissue Metastasized | Genetics | from patient with Down's syndrome | |
| Life Span | infinite | Crisis PDL | |
| Morphology | myeloblast-like | Character | leukemia, acute megakaryoblastic |
| Classify | tumor | Established by | Sato, T. et al. |
| Registered by | Sato,T. | Regulation for Distribution | |
| Comment | This cell line is useful for studying megakaryocytopoiesis. | Year | 1994 |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2665839 | Growth and differentiation of a human megakaryoblastic cell line, CMK. Komatsu N,Suda T,Moroi M,Tokuyama N,Sakata Y,Okada M,Nishida T,Hirai Y,Sato T,Fuse A Blood. 1989 Jul;74(1):42-8 |
| Pubmed id:2527057 | Establishment of a human leukaemic cell line (CMK) with megakaryocytic characteristics from a Down's syndrome patient with acute megakaryoblastic leukaemia. Sato T,Fuse A,Eguchi M,Hayashi Y,Ryo R,Adachi M,Kishimoto Y,Teramura M,Mizoguchi H,Shima Y Br J Haematol. 1989 Jun;72(2):184-90 |
| Pubmed id:2955817 | The c-sis gene expression in cells from a patient with acute megakaryoblastic leukemia and Down's syndrome. Sunami S,Fuse A,Simizu B,Eguchi M,Hayashi Y,Sugita K,Nakazawa S,Okimoto Y,Sato T,Nakajima H Blood. 1987 Aug;70(2):368-71 |
| Pubmed id:3035541 | Molecular cloning of cDNAs of human liver and placenta NADH-cytochrome b5 reductase. Yubisui T,Naitoh Y,Zenno S,Tamura M,Takeshita M,Sakaki Y Proc Natl Acad Sci U S A. 1987 Jun;84(11):3609-13 |
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LOT Information
| Cell No. | IFO50428 | Cell Name | |
|---|---|---|---|
| LOT No. | 02142007 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS lot GIBCO 484375) | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | Dilution. (Small portion of cells becomes adherent in culture without passage.) |
| Doubling Time | 68 hrs | Cell Number in Vial (cells/1ml) | 8.0 x 10^6 |
| Viability at cell freezing (%) | 90 | Antibiotics Used | free |
| Passage Number | Unknown (>4 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:11 D7S820:11,12 D16S539:9,11 VWA:18 TH01:7 AM:X,Y TPOX:9,11 CSF1PO:11,13 |
|
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50428 | Cell Name | CMK-86 |
|---|---|---|---|
| LOT No. | 977 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | dilution | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1 x 10^6 | |
| Viability at cell freezing (%) | 90 | Antibiotics Used | free |
| Passage Number | Unknown (>3 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50428 | Cell Name | CMK-86 |
|---|---|---|---|
| LOT No. | 07282017 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1.5 x 105 cells/mL | Methods for Passages | Simple dilution (suspension culture). The growth at the initiation of culture was slow. |
| Doubling Time | approx. 35 hrs. | Cell Number in Vial (cells/1ml) | 7.6 x 10^5 |
| Viability at cell freezing (%) | 86.9 | Antibiotics Used | free |
| Passage Number | Unknown (3 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 86.9 | Additional information |