IFO50435 KINGS-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50435 | Cell Name | KINGS-1 |
|---|---|---|---|
| Profile | astrocytoma, anaplastic, S-100, vimentin, CD13 and HNK-1-positive, weakly GFAP positive. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 72 |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | anaplastic astrocytoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | mixture of fibroblastic and spherical ce | Character | astrocytoma, anaplastic, GFAP (weak), S-100, vimentin, CD13 and HNK-1-positive |
| Classify | tumor | Established by | Iryo, O. |
| Registered by | Sagawa,K. | Regulation for Distribution | |
| Comment | Year | 1994 | |
| Medium | RPMI1640 medium with 5% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:none | Establishment and characterization of a human glioma cell-line (KINGS-1) carrying leukocytes differentiation antigens. Osamu Iryo J. Kurume Med. Assoc., 51: 304-317 (1988) |
| Images |
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LOT Information
| Cell No. | IFO50435 | Cell Name | KINGS-1 |
|---|---|---|---|
| LOT No. | 1149 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 5-10 x 10^4 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3.0 x 10^6 | |
| Viability at cell freezing (%) | 90 | Antibiotics Used | free |
| Passage Number | Unknown (5 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | MD, G6PD, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50435 | Cell Name | KINGS-1 |
|---|---|---|---|
| LOT No. | 01302014 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 0.5 - 1.6 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 8.6 x 10^5 | |
| Viability at cell freezing (%) | 87.8 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B). MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 87.8 | Additional information |