IFO50473 MEG-01s
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50473 | Cell Name | MEG-01s |
|---|---|---|---|
| Profile | This cell line is useful for studying the differentiation and maturation of megakaryocyts. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 56 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | chronic leukemia | Metastasis | |
| Tissue Metastasized | Genetics | suspension culture adapted subline of MEG-01 | |
| Life Span | infinite | Crisis PDL | |
| Morphology | round | Character | leukemia, chronic megakaryoblastic, MEG-01-derivative |
| Classify | variant | Established by | Ogura, M. et al. |
| Registered by | Ogura,M. | Regulation for Distribution | |
| Comment | Year | 1998 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | Dilution, split ratio 1:4-1:6 |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | bone marrow |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:8954139 | Protein kinase C plays a key role in the cross-talk between intracellular signalings via prostanoid receptors in a megakaryoblastic cell line, MEG-01s. Watanabe T,Sunaga S,Togo M,Satoh H,Higashihara M,Hashimoto Y,Kurokawa K Biochim Biophys Acta. 1996 Nov 22;1304(2):161-9 |
| Pubmed id:7539384 | MEG-01s cells have receptors for and respond to IL-3, IL-6, and SCF. Kellar KL,Hooper WC,Benson JM Exp Hematol. 1995 Jun;23(6):557-64 |
| Pubmed id:1718495 | Characterization of prostaglandin and thromboxane receptors expressed on a megakaryoblastic leukemia cell line, MEG-01s. Watanabe T,Yatomi Y,Sunaga S,Miki I,Ishii A,Nakao A,Higashihara M,Seyama Y,Ogura M,Saito H Blood. 1991 Nov 1;78(9):2328-36 |
| Pubmed id:1367286 | Properties of a cell line (MEG-01SSF) of human megakaryoblastic leukemia cells. Takeuchi M,Satoh M,Ogura M,Saito H,Takeuchi K Res Commun Inst Ferment. 1991;(15):22-9 |
| Pubmed id:2166972 | Enhanced expression of thrombomodulin by intracellular cyclic AMP-increasing agents in two human megakaryoblastic leukemia cell lines. Ito T,Ogura M,Morishita Y,Takamatsu J,Maruyama I,Yamamoto S,Ogawa K,Saito H Thromb Res. 1990 Jun 15;58(6):615-24 |
| Pubmed id:2455575 | Functional and morphological differentiation induction of a human megakaryoblastic leukemia cell line (MEG-01s) by phorbol diesters. Ogura M,Morishima Y,Okumura M,Hotta T,Takamoto S,Ohno R,Hirabayashi N,Nagura H,Saito H Blood. 1988 Jul;72(1):49-60 |
| Pubmed id:2998511 | Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome. Ogura M,Morishima Y,Ohno R,Kato Y,Hirabayashi N,Nagura H,Saito H Blood. 1985 Dec;66(6):1384-92 |
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LOT Information
| Cell No. | IFO50473 | Cell Name | MEG-01s |
|---|---|---|---|
| LOT No. | 1169 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 5 x 10^4 cells/ml | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | 5.3 x 10^6 | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unknown (5 at IFO) | PDL | |
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | NP, G6PD (type B), LD | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:13 D13S317:8 D7S820:11 D16S539:9 VWA:16 TH01:7,8 AM:X,Y TPOX:8,11 CSF1PO:10,11 |
|
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |