IFO50477 FS-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50477 | Cell Name | FS-1 |
|---|---|---|---|
| Profile | This cell line produces IL-6 and SCF into its supernatant. Although this cell line was reported as immortalized cell line, the ampoules preserved in the cell bank hardly proliferated. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | bone marrow stroma |
| Case history | myelogenous leukemia | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | bone marrow stromal cell from a myelogenous leukemia patient |
| Classify | Established by | Kato, H. et al. | |
| Registered by | Kato,H. | Regulation for Distribution | |
| Comment | Although this cell line was reported as immortalized cell line, the ampoules preserved in the cell bank hardly proliferated. | Year | 1998 |
| Medium | Iscove's modified Dulbecco's medium with 20% heat-inactivated fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | 1.6 x 10^5 cells/25-cm2 flask | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | bone marrow stroma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | IFO50477 | Cell Name | FS-1 |
|---|---|---|---|
| LOT No. | 921 | Lot Specification | seed/distribution |
| Medium | Iscove's modification of D.MEM, 80% ; heat-inactivaated FBS, 20% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | tryspin-EDTA, 1.6 x 10^5 cells/25 cm2 flask | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3 x 10^5 | |
| Viability at cell freezing (%) | 88 | Antibiotics Used | free |
| Passage Number | Unknown (5 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:8,11 D7S820:10,12 D16S539:11 VWA:16,18 TH01:6,7 AM:XY TPOX:9,11 CSF1PO:12 |
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| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information | Labeled as S-A R879F921 on vials. |
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