IFO50493 YH-13

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50493 Cell Name   YH-13
Profile   glioblastoma, GFAP and S-100-positive, ACNU(a cancer chemotherapy drug)-resistant Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   40
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   glioblastoma, GFAP and S-100-positive, ACNU (a cancer chemotherapy drug)-resistant
Classify   tumor Established by   Izumi, I. et al.
Registered by   Minemura,K. Regulation for Distribution   
Comment    Year   1998
Medium   Eagle's minimal essential medium with 20% heat-inactivated fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Virus Summary   tested [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Reference
Pubmed id:7947440 Establishment of the two glioma cell lines: YH and AM.
Izumi I,Mineura K,Watanabe K,Kowada M
Hum Cell. 1994 Jun;7(2):101-5
Pubmed id:8145054 Characterization and chemosensitivity of two cell lines derived from human glioblastomas.
Izumu I,Mineura K,Watanabe K,Kowada M
J Neurooncol. 1993 Aug;17(2):111-21

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LOT Information

Cell No.   IFO50493 Cell Name   YH-13
LOT No.   1164 Lot Specification   distribution
Medium   E.MEM, 80%; heat-inactivated FBS, 20% Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5.6 x 10^5
Viability (%)   84 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, PEP B, AST, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Additional information   
Cell No.   IFO50493 Cell Name   YH-13
LOT No.   02242014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.5 - 3.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.5 x 10^5
Viability (%)   98.8 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSo, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Additional information   
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