JCRB0001 F9-41
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0001 | Cell Name | F9-41 |
|---|---|---|---|
| Profile | Mouse testicular teratocarcinoma cell line. | Other Name | F9 |
| Animal | mouse | Strain | 129 |
| Genus | Mus | Species | musculus |
| Sex | M | Age | |
| Identity | not done | Tissue for Primary Cancer | teratoma |
| Case history | testicular teratocarcinoma | Metastasis | No |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | cells are differentiated by retinoic acit or cAMP |
| Classify | transformed | Established by | Bernstine et al. |
| Registered by | Matsumura,T. | Regulation for Distribution | Not regulated |
| Comment | Maximum cell density=1-2x10^7/10D. Do not culture more than 1 month. | Year | 1985 |
| Medium | Dulbecco's modified Eagle's medium with 15% fetal calf serum. | Methods for Passages | Harvest cells after treatment with 0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely before scoring cell number. |
| Cell Number on Passage | 1.5 x 10^6 cells/100mm dish | Race | NT |
| CO2 Conc. | 5 % | Tissue Sampling | testis |
| Tissue Type | teratocarcinoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1706663 | Characterization of a murine beta 1-4 galactosyltransferase expressed in COS-1 cells. Nakazawa K,Furukawa K,Kobata A,Narimatsu H Eur J Biochem. 1991 Mar 14;196(2):363-8 |
| Pubmed id:6164928 | Cell interactions modulate embryonal carcinoma cell differentiation into parietal or visceral endoderm. Hogan BL,Taylor A,Adamson E Nature. 1981 May 21;291(5812):235-7 |
| Pubmed id:6250719 | Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Strickland S,Smith KK,Marotti KR Cell. 1980 Sep;21(2):347-55 |
| Pubmed id:214238 | The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Strickland S,Mahdavi V Cell. 1978 Oct;15(2):393-403 |
| Pubmed id:1103673 | [Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)]. Nicolas JF,Dubois P,Jakob H,Gaillard J,Jacob F Ann Microbiol (Paris). 1975 Jan;126(1):3-22 |
| Pubmed id:4521215 | Alkaline phosphatase activity in mouse teratoma. Berstine EG,Hooper ML,Grandchamp S,Ephrussi B Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899-903 |
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LOT Information
| Cell No. | JCRB0001 | Cell Name | F9-41 |
|---|---|---|---|
| LOT No. | 022294 | Lot Specification | distribution |
| Medium | Dalbecco's modified Eagle's medium with 15% fetal bovine serum (J.R.S. lot.DO10400) | Temperature | 37 C |
| Cell Density at Seeding | 1.5x10^5 cells/ml | Methods for Passages | Wash cells with PBS(-) and treated with 0.125%-trypsin and 0.01% EDTA for 5-10 minutes. |
| Doubling Time | 22.6Hrs. | Cell Number in Vial (cells/1ml) | 1.15x10^7 |
| Viability at cell freezing (%) | 87.6 | Antibiotics Used | free |
| Passage Number | P7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | LD, NP (G6PD not detected) |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0001 | Cell Name | F9-41 |
|---|---|---|---|
| LOT No. | 041690 | Lot Specification | distribution |
| Medium | Dulbecco`s modified Eagle's medium with 15% fetal calf serum (10% can be applicable). | Temperature | 37 C |
| Cell Density at Seeding | 1.5X10^6/10D | Methods for Passages | 0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.3x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P6* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0001 | Cell Name | F9-41 |
|---|---|---|---|
| LOT No. | 051288 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 15% fetal calf serum | Temperature | 37 C |
| Cell Density at Seeding | 1.5X10^5 cells/ml | Methods for Passages | 0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely. |
| Doubling Time | D.T.=10.27hr | Cell Number in Vial (cells/1ml) | 8.2x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P5* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | LD,NP,G6PD |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
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