JCRB0023 RBL-2H3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0023 Cell Name   RBL-2H3
Profile   Famous rat mast cell line releasing IgE mediated histamine, established from peripheral blood with basophilic leukemia. Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   basophilic leukemia Metastasis   
Tissue Metastasized    Genetics   mast cell line
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   IgE mediated histamine release was reported.
Classify   tumor Established by   Siraganian,R.
Registered by   Siraganian, R. Regulation for Distribution   Allowed to use only for the research purpose.
Comment   Release IgE-mediated serotin. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   5x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0591
Reference
Pubmed id:10731674Rapid turnover of tryptophan hydroxylase is driven by proteasomes in RBL2H3 cells, a serotonin producing mast cell line.
Kojima M,Oguro K,Sawabe K,Iida Y,Ikeda R,Yamashita A,Nakanishi N,Hasegawa H
J Biochem. 2000 Jan;127(1):121-7
Pubmed id:7615072Rapid turnover of tryptophan hydroxylase in serotonin producing cells: demonstration of ATP-dependent proteolytic degradation.
Hasegawa H,Kojima M,Oguro K,Nakanishi N
FEBS Lett. 1995 Jul 10;368(1):151-4
Pubmed id:7721878Regulation of the adapter molecule Grb2 by the Fc epsilon R1 in the mast cell line RBL2H3.
Turner H,Reif K,Rivera J,Cantrell DA
J Biol Chem. 1995 Apr 21;270(16):9500-6
Pubmed id:7513514Cell proliferation status, cytokine action and protein tyrosine phosphorylation modulate leukotriene biosynthesis in a basophil leukaemia and a mastocytoma cell line.
Hagmann W
Biochem J. 1994 Apr 15;299 ( Pt 2)():467-72
Pubmed id:2540803Implanted IgE-Fc epsilon R complexes elicit IgE-mediated activation of RBL-2H cells.
Ran S,Loyter A,Rivnay B
Biochemistry. 1989 Jan 24;28(2):644-51
Pubmed id:2642390Receptor-mediated release of inositol 1,4,5-trisphosphate and inositol 1,4-bisphosphate in rat basophilic leukemia RBL-2H3 cells permeabilized with streptolysin O.
Ali H,Cunha-Melo JR,Beaven MA
Biochim Biophys Acta. 1989 Jan 17;1010(1):88-99
Pubmed id:3262612Formation of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate from inositol 1,3,4,5-tetrakisphosphate and their pathways of degradation in RBL-2H3 cells.
Cunha-Melo JR,Dean NM,Ali H,Beaven MA
J Biol Chem. 1988 Oct 5;263(28):14245-50
Pubmed id:2845404Cholera toxin potentiates IgE-coupled inositol phospholipid hydrolysis and mediator secretion by RBL-2H3 cells.
McCloskey MA
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7260-4
Pubmed id:2462904Isolation and purification of an Fc epsilon receptor activated ion channel from the rat mast cell line RBL-2H3.
Hemmerich S,Pecht I
Biochemistry. 1988 Sep 20;27(19):7488-98
Pubmed id:6168684Inhibition of IgE-mediated histamine release from rat basophilic leukemia cells and rat mast cells by inhibitors of transmethylation.
Morita Y,Siraganian RP
J Immunol. 1981 Oct;127(4):1339-44
Pubmed id:6166481IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones.
Barsumian EL,Isersky C,Petrino MG,Siraganian RP
Eur J Immunol. 1981 Apr;11(4):317-23
Pubmed id:4812630The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE.
Kulczycki A Jr,Isersky C,Metzger H
J Exp Med. 1974 Mar 1;139(3):600-16
Pubmed id:4516144Basophilic leukaemia in the albino rat and a demonstration of the basopoietin.
Eccleston E,Leonard BJ,Lowe JS,Welford HJ
Nat New Biol. 1973 Jul 18;244(133):73-6

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LOT Information

Cell No.   JCRB0023 Cell Name   
LOT No.   04082011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   09062010 Lot Specification   distribution
Medium   MEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.0-7.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.31x10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0023 Cell Name   
LOT No.   04102009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA. Hardly to detach and vigorous pipetting is needed. Passage every 3-4 days.
Doubling Time   21 - 23 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   
LOT No.   02012008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS Lot; 484345) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.3 days Cell Number in Vial (cells/1ml)   5.4 x 10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confrimed as rat by G6PD, NP MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   
LOT No.   02132007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484735) Temperature   37 C
Cell Density at Seeding   1 - 4 x 10^4 cells/sq.cm Methods for Passages   Treat the cells with 0.25% trypsin and 0.02% EDTA after washing with PBS(-) for 3 times.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   6.2 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (P5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   
LOT No.   05182005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO4218333S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA. Hardly to detach and vigorous pipetting is needed. Passage every 3-4 days.
Doubling Time   approx. 1day Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   P5 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   
LOT No.   07112003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA. Hardly to detach and vigorous pipetting is needed. Passage every 3-4 days.
Doubling Time   D.T. = ca. 1.4 days Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   90.5 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM) CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   
LOT No.   06142000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   3-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO and 20% FCS CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   020596 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (Flow Labs.0070893) Temperature   37 C
Cell Density at Seeding   1-5x10^5 cells/ml Methods for Passages   Wash cells with PBS(-), and treat with 0.25% trypsine and 0.02% EDTA for 3-10 min at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH,G6PD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   012894 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (ICN Flow 101083) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   0.25% trypsin & 0.02% EDTA then scrape by rubber policeman. (1% collagenase type IX not effective)
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.24x10^5
Viability at cell freezing (%)   89.1 Antibiotics Used   free
Passage Number   P4 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, G6PD, NP, by Okado,K. Feb.16.'94
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   041892 Lot Specification   distribution
Medium   MEM (lot.032392) + 10% FBS (Cell Cult.Lab.00939-01) Temperature   37 C
Cell Density at Seeding   5x10^4 cells/ml Methods for Passages   0.25 % trypsin in 0.02 % EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.19x10^6
Viability at cell freezing (%)   80.3 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   112889 Lot Specification   distribution
Medium   MEM + FCS 10% Temperature   37
Cell Density at Seeding   5x10^4 cells/ml Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   092587 Lot Specification   distribution
Medium   MEM + FCS 10% Temperature   37
Cell Density at Seeding   5x10^4 cells/ml Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8x10^5
Viability at cell freezing (%)   81% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   
LOT No.   091897 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   40 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   06142018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Hardly to detach and vigorous pipetting is needed. Passage every 3-4 days.
Doubling Time   approx. 16 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93.9 Additional information   
Cell No.   JCRB0023 Cell Name   RBL-2H3
LOT No.   08242023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322). Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 18 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98.0 Additional information   
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