JCRB0025 BRL-3A

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0025 Cell Name   BRL-3A
Profile   MSA (somatomedin-like) protein producing, (Buffalo rat) Other Name   
Animal   rat Strain   Buffalo
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   liver
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Producing MSA protein (somatomedin-like).
Classify   transformed Established by   Coon,H.G.
Registered by   Suzuki,K. Regulation for Distribution   Free
Comment   Production of somatomedin. Year   1988
Medium   Ham's F12 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0606
Reference
Pubmed id:4308097A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.
Coon HG,Weiss MC
Proc Natl Acad Sci U S A. 1969 Mar;62(3):852-9

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LOT Information

Cell No.   JCRB0025 Cell Name   BRL-3A
LOT No.   031495 Lot Specification   FDSC/distribution
Medium   Ham's F12 medium (Gibco) with 10% fetal bovine serum (Bio Cell lot.4001776) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/ml Methods for Passages   Treat cells by 0.07% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.94x10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0025 Cell Name   BRL-3A
LOT No.   112291 Lot Specification   distribution
Medium   Ham's F12 + FCS 10% Temperature   37
Cell Density at Seeding   2.4x10^6 cells/ml Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time   ND Cell Number in Vial (cells/1ml)   3.6x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P5* PDL   ND
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0025 Cell Name   BRL-3A
LOT No.   052588 Lot Specification   distribution
Medium   Ham's F12 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 dilution Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   D.T.=19.8hr Cell Number in Vial (cells/1ml)   1.2X10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0025 Cell Name   BRL-3A
LOT No.   051388 Lot Specification   distribution
Medium   Ham's F12 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 dilution Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   D.T.=19.8hr Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0025 Cell Name   
LOT No.   072398 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2-4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   48 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^5
Viability at cell freezing (%)   81.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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