JCRB0030 CHL/IU(IVGT)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0030 Cell Name   CHL/IU(IVGT)
Profile   Chinese hamster lung cells. Standard cell line for chromosome aberation test. Other Name   CHL
Animal   hamster, Chinese Strain   Chinese
Genus   Cricetulus Species   griseus
Sex   F Age   new born
Identity    Tissue for Primary Cancer   lung
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   A clone of normal chinese hamster lung(CHL) cell line
Classify   transformed Established by   Utakoji,T.
Registered by   Ishidate,M. Regulation for Distribution   Free
Comment   Useful for chromosomal aberration tests in vitro as a screening tool for the detection of possible carcinogenic substances. Year   1985
Medium   Eagle's minimal essential medium with 10 % calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin. Subculture every 3-4 days.
Cell Number on Passage   10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_GP67
Reference
Pubmed id:876270Chromosome tests with 134 compounds on Chinese hamster cells in vitro--a screening for chemical carcinogens.
Ishidate M Jr,Odashima S
Mutat Res. 1977 Jul;48(3-4):337-53
Pubmed id:5452405A new cell line derived from newborn Chinese hamster lung tissue.
Koyama H,Utakoji T,Ono T
Gan. 1970 Apr;61(2):161-7

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LOT Information

Cell No.   JCRB0030 Cell Name   CHL/IU(IVGT)
LOT No.   05162005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum. Labelled as "CHL/IU" on vial. Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture with 3-4 days interval.
Doubling Time   approx. 20 hrs Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   76.8 Antibiotics Used   free
Passage Number   P6 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - Eagle's MEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0030 Cell Name   CHL/IU(IVGT)
LOT No.   113092 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum (Cell culture Laboratories 00021-01) Temperature   37 C
Cell Density at Seeding   9.4 x 10^5 cells/ml Methods for Passages   Cells are washed once with PBS(-) and treated with0.25% trypsin and 0.02% EDTA.
Doubling Time   17.6hrs,6/26/95 Cell Number in Vial (cells/1ml)   4.67x 10^6
Viability at cell freezing (%)   94.1 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP,G6PD
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0030 Cell Name   CHL/IU(IVGT)
LOT No.   072487 Lot Specification   distribution
Medium   MEM + CS 10% Temperature   37
Cell Density at Seeding   10^5 cells/60 mm dish Methods for Passages   0.02% EDTA and 0.25% Trypsin. Subculture every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5x10^5
Viability at cell freezing (%)   85% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0030 Cell Name   CHL/IU(IVGT)
LOT No.   06032013 Lot Specification   distribution
Medium   MEM medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.12-3.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   Labeled as CHL/IU
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Cell No.   JCRB0030 Cell Name   CHL/IU(IVGT)
LOT No.   070197 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum. Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA, subculture every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   86.0 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   (Culture medium with 5% DMSO) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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