JCRB0042 HEC-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0042 Cell Name   HEC-1
Profile   Human endometrial adenocarcimona cell line. (HEC-1-B). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   71
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   
Tissue Metastasized    Genetics   STR analysis in the cell bank indicated that the HEC-1 is the same cell line as the HEC-1-B (NIHS0480).
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Kuramoto,H.
Registered by   Sofuni,T. Regulation for Distribution   
Comment   Mycoplasma contamination was detected when submitted to NIHS and eliminated at 1986. Year   1985
Medium   Eagle's minimal essential medium with 10% calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/10 - 1/3 Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   endometrial adenocarcimona

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1274
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907051
Reference
Pubmed id:15053063Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60
Pubmed id:6299971Insensitivity to interferon of two subclones of human endometrial carcinoma cell line, HEC-1.
Morinaga N,Yonehara S,Tomita Y,Kuwata T
Int J Cancer. 1983 Jan 15;31(1):21-8
Pubmed id:862644Establishment and characterization of the cell-line of a human edometrial adenoacanthoma.
Kuramoto H,Hamano M
Eur J Cancer. 1977 Mar 29;13(3):253-9
Pubmed id:269609Cytogenetic studies of human endometrial carcinomas by means of tissue culture.
Kuramoto H,Hamano M
Acta Cytol. 1977 Jul-Aug;21(4):559-65

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LOT Information

Cell No.   JCRB0042 Cell Name   
LOT No.   12142012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   8.3 x 10^5
Viability at cell freezing (%)   92.9 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0042 Cell Name   HEC-1
LOT No.   08172010 Lot Specification   distribution
Medium   MEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.7-2.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.21x10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   p15* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11
D7S820:11
D16S539:11,12
VWA:18
TH01:6,7
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0042 Cell Name   
LOT No.   111398 Lot Specification   distribution
Medium   Eagle's minimal essential medium + 10% calf serum (Biowhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   5.0 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 40 hrs Cell Number in Vial (cells/1ml)   7.4 x 10^5
Viability at cell freezing (%)   92% Antibiotics Used   free
Passage Number   P13* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD(type B), LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0042 Cell Name   HEC-1
LOT No.   031593 Lot Specification   distribution
Medium   Eagle's minimal essential medium with (10%) calf serum (ICN Flow 1859823) Temperature   37 C
Cell Density at Seeding   8.0x10^4 cells/ml Methods for Passages   Wash cells with PBS and treated with 0.02% EDTA and 0.2% trypsin at room temp. for a few second.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   88.7 Antibiotics Used   free
Passage Number   P14 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(Btype),LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0042 Cell Name   HEC-1
LOT No.   101389 Lot Specification   distribution
Medium   MEM + CS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 - 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P13* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0042 Cell Name   HEC-1
LOT No.   042686 Lot Specification   distribution
Medium   MEM + CS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 - 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8x10^5/ml
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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