JCRB0054 LU65A
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0054 | Cell Name | LU65A |
|---|---|---|---|
| Profile | giant cell carcinoma | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 64 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | lung cancer | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | tumor | Established by | Suzuki,K. et al. |
| Registered by | Suzuki,K. | Regulation for Distribution | Permission for the distribution required. |
| Comment | Year | 5 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25 % trypsin. |
| Cell Number on Passage | split ratio=1/10 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type | giant cell carcinoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2112525 | A simple, general method for detecting retroviral RNAs expressed in cells. Harada F,Hirose Y,Tanaka M,Sasaki T,Kamiya H,Miura K Jpn J Cancer Res. 1990 Mar;81(3):232-7 |
| Pubmed id:2659722 | Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells. Suzuki K,Miyasaka H,Ota H,Yamakawa Y,Tagawa M,Kuramoto A,Mizuno S J Exp Med. 1989 Jun 1;169(6):1895-901 |
| Pubmed id:3935627 | Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo. Yamada T,Hirohashi S,Shimosato Y,Kodama T,Hayashi S,Ogura T,Gamou S,Shimizu N Jpn J Cancer Res. 1985 Oct;76(10):967-76 |
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LOT Information
| Cell No. | JCRB0054 | Cell Name | LU65A |
|---|---|---|---|
| LOT No. | 040795 | Lot Specification | FDSC/distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (Bio Cell, 4001776). Lot # labeled on Ampoule is wrong. Correct lot # is 040795. | Temperature | 37 C |
| Cell Density at Seeding | 7.8 - 17.9x10^4 cells/ml | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA mix. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 9.5x10^5 |
| Viability at cell freezing (%) | 93.8 | Antibiotics Used | free |
| Passage Number | P14 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information | Lot# 040759 on vials is typo. The correct lot# is 040795. |
| Cell No. | JCRB0054 | Cell Name | LU65A |
|---|---|---|---|
| LOT No. | 111789 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal calf serum | Temperature | 37 |
| Cell Density at Seeding | dilution 1/10 | Methods for Passages | 0.02% EDTA and 0.05% Trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.1x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P14 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0054 | Cell Name | LU65A |
|---|---|---|---|
| LOT No. | 102885 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | dilution 1/10 | Methods for Passages | 0.02% EDTA and 0.05% Trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P15 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | AST,G6PD,LD,MD,MPI,NP,PEPB |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |