JCRB0062 HEL

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0062 Cell Name   HEL
Profile   leukemia, erythroid Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Martin,P.
Registered by   Akiyama,M. Regulation for Distribution   free
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/3 Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   erythroid leukemia

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0001
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907053
Reference
Pubmed id:3052623Platelet glycoprotein IIb/IIIa complex in cultured cells. Localization in focal adhesion sites in spreading HEL cells.
Ylänne J,Hormia M,Järvinen M,Vartio T,Virtanen I
Blood. 1988 Nov;72(5):1478-86
Pubmed id:2436686A monoclonal antibody reacting with human basophils.
Bodger MP,Mounsey GL,Nelson J,Fitzgerald PH
Blood. 1987 May;69(5):1414-8

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LOT Information

Cell No.   JCRB0062 Cell Name   
LOT No.   06152000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   6 x 10^4 cells/ml Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Doubling Time   ca 22 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 10% DMSO and 20% FCS CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   033095 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated serum bovine serum (J.R.H.B. 1B1062) Temperature   37 C
Cell Density at Seeding   1.3-1.8x10^4 cells/sq.cm. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA at 37 C for 3 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.37x10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   Karyotyme examined. Data available.
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   011293 Lot Specification   distribution
Medium   RPMI1640 medium(090892) with 10% fetal bovine serum (JRHB lot1B1062) Temperature   37 C
Cell Density at Seeding   1.3x10^5 cells/ml Methods for Passages   Wash once with PBS and treated with 0.02% EDTA and 0.25% trypsin at 37 C for 15 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   050891 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   013088 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   102986 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   02192015 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.8 - 3.7 x 10-5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25 % trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:9,11
D7S820:7
D16S539:11
VWA:14,17
TH01:7
AM:X
TPOX:11
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   11192018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.8 - 2.9 x 10^5 clls/mL Methods for Passages   Cells were harvested after treatment with 0.25 % trypsin and 0.02% EDTA.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   85.2 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85.2 Additional information   
Cell No.   JCRB0062 Cell Name   HEL
LOT No.   07102024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.6 - 3.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25 % trypsin and 0.02% EDTA. (HEL cells are adherent cells).
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
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