JCRB0083 4-8H

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0083 Cell Name   4-8H
Profile   anti-DNA polymerase alpha, IgG1 Other Name   4-8H
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   
Tissue Metastasized    Genetics   hybridoma
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   antibody to DNA polymerase alpha, IgG1
Classify   hybridoma Established by   Matsukage,A.
Registered by   Matsukage,A. Regulation for Distribution   Report requested on distribution.
Comment   Everyday medium change is necessary for good proliferation. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   Less than 10^6 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   NS1 x spleen cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J272
Reference
Pubmed id:2415123Monoclonal antibody specific for chicken DNA polymerase alpha associated with DNA primase.
Hirose F,Kedar P,Takahashi T,Matsukage A
Biochem Biophys Res Commun. 1985 Oct 15;132(1):210-6
Pubmed id:7160577Establishment of hybridoma clones which produce anti-chick embryo DNA polymerase alpha monoclonal antibodies.
Matsukage A,Yamaguchi M,Tanabe K,Nishizawa M,Takahashi T,Seto M,Takahashi T
Gan. 1982 Dec;73(6):850-3




LOT Information

Cell No.   JCRB0083 Cell Name   4-8H
LOT No.   041886 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   less than 10^6/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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