JCRB0084 4-2D

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0084 Cell Name   4-2D
Profile   anti-primase complexed with DNA polymerase alpha Other Name   4-2D
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   
Tissue Metastasized    Genetics   hybridoma, producing IgG antibody.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   antibody to primase complexed withDNA polymerase a
Classify   hybridoma Established by   Matsukage,A.
Registered by   Matsukage,A. Regulation for Distribution   Restricted only for the cooperative research with Dr.Matsukage.
Comment   Everyday medium change is necessary for good prolife-ration. Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   Less than 10^6 cells/ml. Race   
CO2 Conc.   5 % Tissue Sampling   NS1 x spleen cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J273
Reference
Pubmed id:2415123Monoclonal antibody specific for chicken DNA polymerase alpha associated with DNA primase.
Hirose F,Kedar P,Takahashi T,Matsukage A
Biochem Biophys Res Commun. 1985 Oct 15;132(1):210-6
Pubmed id:6355128Immunocytochemical localization of chick DNA polymerases alpha and beta +.
Matsukage A,Yamamoto S,Yamaguchi M,Kusakabe M,Takahashi T
J Cell Physiol. 1983 Nov;117(2):266-71
Pubmed id:7160577Establishment of hybridoma clones which produce anti-chick embryo DNA polymerase alpha monoclonal antibodies.
Matsukage A,Yamaguchi M,Tanabe K,Nishizawa M,Takahashi T,Seto M,Takahashi T
Gan. 1982 Dec;73(6):850-3




LOT Information

Cell No.   JCRB0084 Cell Name   4-2D
LOT No.   061286 Lot Specification   distribution
Medium   RPMI1640+ FCS 10% Temperature   37
Cell Density at Seeding   less than 10^6 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P11* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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