JCRB0106 SCCH-26

Cell information

Important Notice(s)

ES medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0106 Cell Name   SCCH-26
Profile   Human neuroblastoma cell line. Other Name   (eliminated)
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   No
Tissue Metastasized    Genetics   neuroblastoma
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   neuroblastoma
Classify   tumor Established by   Kaneko,Y.
Registered by   Kaneko,Y. Regulation for Distribution   Not regulated
Comment   Other name was discarded because of the personal ID. Year   1987
Medium   ES medium with 10% calf serum. The ES medium is Eagle's MEM (containing L-glutamine) supplemented with 0.2 mM each non essential amino acids, 1 mM sodium pyruvate, and 0.1 mg/L cyanocobalamin (vitamine B12). Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes EBV(low copy)

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1686
Reference
Pubmed id:2121721Monoclonal antibodies to disialogangliosides: characterization of antibody-mediated cytotoxicity against human melanoma and neuroblastoma cells in vitro.
Kawashima I,Tada N,Fujimori T,Tai T
J Biochem. 1990 Jul;108(1):109-15
Pubmed id:7105014Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate.
Koyama H,Kodama H
Cancer Res. 1982 Oct;42(10):4210-4

Images



LOT Information

Cell No.   JCRB0106 Cell Name   SCCH-26
LOT No.   101795 Lot Specification   distribution
Medium   ES medium with 10% fetal bovine serum (JRHB B1B1062) Temperature   37 C
Cell Density at Seeding   4.0x10^5 cells/sq.cm. Methods for Passages   Mechanical dispersion by pipetting
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.07x10^6
Viability at cell freezing (%)    Antibiotics Used   Kanamycine 60ug/ml
Passage Number   P9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% dimethyl sulfoxide (DMSO) CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0106 Cell Name   SCCH-26
LOT No.   033092 Lot Specification   distribution
Medium   ES medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1x10^6 cells/ml Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   3.6x10^6
Viability at cell freezing (%)   68.2 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0106 Cell Name   SCCH-26
LOT No.   102590 Lot Specification   distribution
Medium   ES + FCS 10% Temperature   37
Cell Density at Seeding   split ratio:1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0106 Cell Name   SCCH-26
LOT No.   111987 Lot Specification   distribution
Medium   ES + FCS 10% Temperature   37
Cell Density at Seeding   split ratio:1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8x10^5
Viability at cell freezing (%)   65% Antibiotics Used   KM
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0106 Cell Name   
LOT No.   03082000 Lot Specification   distribution
Medium   ES medium (Nissui Seiyaku) with 10% calf serum Temperature   37 C
Cell Density at Seeding   6-14 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Growth retards at high cell density. Passage at 30-50% confluency.
Doubling Time   3 days Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   53 Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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