JCRB0110 M1-T22

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0110 Cell Name   M1-T22
Profile   Spontaneously occured myeloid leukemia cell line differentiated by dexamethasone or etc. Other Name   M1 clone T22
Animal   mouse Strain   SL
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   spontaneous myeloid leukemia Metastasis   
Tissue Metastasized    Genetics   cytologically determined as myeloblast
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Cells are differentiated by dexamethasone or etc.
Classify   tumor Established by   Ichikawa,Y.
Registered by   Hozumi,M. Regulation for Distribution   Not regulated.
Comment    Year   1987
Medium   Eagle's minimal essential medium with x2 amino acids-vitamins and 10% fetal calf serum Methods for Passages   Dilute every 3 days.
Cell Number on Passage   1-2x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8368
Reference
Pubmed id:4517230Spontaneous development of macrophage-like cells in a culture of myeloid leukemia cells.
Maeda M,Ichikawa Y
Gan. 1973 Jun;64(3):265-71
Pubmed id:4187945Differentiation of a cell line of myeloid leukemia.
Ichikawa Y
J Cell Physiol. 1969 Dec;74(3):223-34




LOT Information

Cell No.   JCRB0110 Cell Name   M1-T22
LOT No.   051592 Lot Specification   distribution
Medium   MEM + AA with vitamin (lot#.041592) + FBS 10% (lot. Cell Cult.Labs.00939-01) Temperature   37 C
Cell Density at Seeding   1-2x10^6 cells/ml Methods for Passages   dilution (dilute to 1-2x10^5 cells/ml with 3-4 days interval). Minimum inoculum size=2x10^4cells/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   P15* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0110 Cell Name   M1-T22
LOT No.   110788 Lot Specification   distribution
Medium   MEM w/ x2 amino acids, vitamins + FCS 10% Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Dilute every 3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P14* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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