JCRB0115 SCC-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0115 Cell Name   SCC-3
Profile   Human monocytic cell line expressing Interleukin-2 receptor. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   20
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   non Hodgkin's lymphoma, subc. tumors of breast. Metastasis   Yes
Tissue Metastasized   pleural effusion Genetics   LSG type. IL2 receptors were expressed.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   monocytic cell line. Negative for ATLA. Positive for EBVCA and negative for EBNA.
Classify   tumor Established by   Kimura,Y.
Registered by   Kimura,Y. Regulation for Distribution   Not defined.
Comment    Year   1988
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1683
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910930
Reference
Pubmed id:3095281Establishment and characterization of a monocytic cell line which expresses the interleukin-2 receptor.
Kimura Y,Toki H,Okabe K,Suzuki K,Hayakawa T,Miyamoto K
Jpn J Cancer Res. 1986 Sep;77(9):862-5

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LOT Information

Cell No.   JCRB0115 Cell Name   SCC-3
LOT No.   012194 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat inactivated fetal vobine serum (JRS.DO10400) Temperature   37 C
Cell Density at Seeding   2-3x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.7x10^6
Viability at cell freezing (%)   81.2 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,PepB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9
D13S317:8
D7S820:11,12
D16S539:12
VWA:16,17
TH01:9
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0115 Cell Name   SCC-3
LOT No.   052291 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0115 Cell Name   SCC-3
LOT No.   01242024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   4 - 6 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
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