JCRB0118 SKM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0118 Cell Name   SKM-1
Profile   myelo dysplastic syndrome Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   76
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   myelo dysplastic syndrome. Metastasis   No
Tissue Metastasized    Genetics   MDS->RAEB.T->AML
Life Span   infinite Crisis PDL   NT
Morphology   lymphocyte-like Character   Myeloperoxidase (MPO) release into cult.medium.
Classify   transformed Established by   Kawaguchi,R.
Registered by   Kawaguchi,R. Regulation for Distribution   
Comment    Year   1993
Medium   RPMI1640 medium with 10% or 20% heat inactivated fetal bovine serum. Methods for Passages   Simple dilution because of suspension cell culture.
Cell Number on Passage   2.0 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0098
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909722
Reference
Pubmed id:8383257Cloning and characterization of four types of cDNA encoding myeloperoxidase from human monocytic leukemia cell line, SKM-1.
Hosokawa Y,Kawaguchi R,Hikiji K,Yamada M,Suzuki K,Nakagawa T,Yoshihara T,Yamaguchi K
Leukemia. 1993 Mar;7(3):441-5
Pubmed id:2004029Loss of multiple point mutations of RAS genes associated with acquisition of chromosomal abnormalities during disease progression in myelodysplastic syndrome.
Nakagawa T,Saitoh S,Imoto S,Itoh M,Tsutsumi M,Hikiji K,Nakao Y,Fujita T
Br J Haematol. 1991 Feb;77(2):250-2

Images



LOT Information

Cell No.   JCRB0118 Cell Name   SKM-1
LOT No.   03072012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.4 - 2.6 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   80.8 Antibiotics Used   free
Passage Number   89 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   91% Additional information   
Cell No.   JCRB0118 Cell Name   SKM-1
LOT No.   043099 Lot Specification   distribution
Medium   RPMI1640 medium with 10-20% heat inactivated fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.3 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension cell culture).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Under examination.
Chromosome Mode    Chromosome Information   Chromosome analyzed.
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culturemedium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0118 Cell Name   SKM-1
LOT No.   03052021 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.29x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.16x10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:11,13
D7S820:10,11
D16S539:9,11
VWA:18
TH01:7,9
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
Images
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