JCRB0123 KO52

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0123 Cell Name   KO52
Profile   leukemia, acute myeloblastic Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   46
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute myeloblastic leukemia/M2 Metastasis   No
Tissue Metastasized    Genetics   CD12,CD33,CD2,CD5,CD7,HLA-DR. chr=17p-, MDR1 exp.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   N-ras mutation: G->C in codon 13.
Classify   tumor Established by   Abo,J. & Inokuchi,K. et.al.
Registered by   Inokuchi,K. Regulation for Distribution   
Comment    Year   1997
Medium   Alpha-MEM with 10% heat inactivated fetal calf serum . Methods for Passages   Simple dilution (suspension culture).
Cell Number on Passage   2.5 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1321
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1330932
Reference
Pubmed id:7693050p53 and N-ras mutations in two new leukemia cell lines established from a patient with multilineage CD7-positive acute leukemia.
Abo J,Inokuchi K,Dan K,Nomura T
Blood. 1993 Nov 1;82(9):2829-36

Images



LOT Information

Cell No.   JCRB0123 Cell Name   KO52
LOT No.   11242010 Lot Specification   distribution
Medium   Alpha-MEM (minimum essential medium alpha) with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Simple dilution
Doubling Time   52 - 58 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6 cells/ml
Viability at cell freezing (%)   88.5 Antibiotics Used   free
Passage Number   Unkown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - alpha-MEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0123 Cell Name   KO52
LOT No.   081497 Lot Specification   distribution
Medium   Alpha-MEM with 10% heat inactivated fetal calf serum (JRH Bio Sciences 4B2013). Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture).
Doubling Time   See graph. Cell Number in Vial (cells/1ml)   3.2x10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,12
D7S820:9,10
D16S539:9,11
VWA:16
TH01:6,7
AM:XY
TPOX:8
CSF1PO:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0123 Cell Name   KO52
LOT No.   04082016 Lot Specification   distribution
Medium   Alpha-MEM with 10% heat inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). In the first cultivation, do not excessivly dilute. Please seed all cells to one T-25 flask (or one 6 cm dish).
Doubling Time   approx. 61 hrs. Cell Number in Vial (cells/1ml)   6.9 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:8,12
D7S820:9,10
D16S539:9,11
VWA:16
TH01:6,7
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - alpha-MEM CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   Don't dilute too much on the first culture so the cell death may be easily caused.
Cell No.   JCRB0123 Cell Name   KO52
LOT No.   05272016 Lot Specification   distribution
Medium   Alpha-MEM with 10% heat inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   81 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   5% DMSO, 20% HI-FBS - alpha MEM CO2 Conc.   5%
Viability immediately after thawing (%)   81 Additional information   
Cell No.   JCRB0123 Cell Name   KO52
LOT No.   07142021 Lot Specification   distribution
Medium   Alpha-MEM with 10% fetal bovine serum (FBS; Biowest, Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.4 x 4.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 2 days. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - alpha-MEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.8 Additional information   
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