JCRB0126.1 KOSC-2 cl3-43

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0126.1 Cell Name   KOSC-2 cl3-43
Profile   Human tumor cells derived from oral cancer at stage III. The cells were originally cross contaminated with mouse cells but the mouse cells were eliminated in the JCRB cell bank and the cl3-43 subclone ID was applied to the cell name. Other Name   KOSC-2
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   51
Identity   available Tissue for Primary Cancer   oral
Case history   oral cancer at stage III. Mouse cells eliminated by cell cloning & mycoplasmas eliminated by MC210. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Moderately differentiated squamous-cell carcinoma.
Classify   tumor Established by   Inagaki,T.
Registered by   Inagaki,T. Regulation for Distribution   free
Comment   Cells were established through xenografted tumors in nude mice by Geneticin treatment to eliminate mouse fibroblast cells. Mouse cells observed when deposited. Year   1998
Medium   RPMI-1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are passaged twice a week by trypsinization.
Cell Number on Passage   2.2 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   oral floor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1337
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 753570
Reference
Pubmed id:8314315Establishment of human oral-cancer cell lines (KOSC-2 and -3) carrying p53 and c-myc abnormalities by geneticin treatment.
Inagaki T,Matsuwari S,Takahashi R,Shimada K,Fujie K,Maeda S
Int J Cancer. 1994 Jan 15;56(2):301-8

Images



LOT Information

Cell No.   JCRB0126.1 Cell Name   KOSC-2 cl3-43
LOT No.   08222000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   7.0 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temerature for 5 minutes. Change medium every 2-3 days and subculture every 5 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   free
Passage Number   P11 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typB), NP, LDH examined by Sasaki,A. (human)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9
D13S317:11
D7S820:10,11
D16S539:9,12
VWA:15,17
TH01:6,7
AM:X
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0126.1 Cell Name   KOSC-2 cl3-43
LOT No.   08102000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   7.0 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Change medium every 2-3 days and subculture every 5 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(Type B), LDH, NP examined. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9
D13S317:11
D7S820:10,11
D16S539:9,12
VWA:15,17
TH01:6,7
AM:X
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0126.1 Cell Name   KOSC-2 cl3-43
LOT No.   10172013 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. #10099). Temperature   37C
Cell Density at Seeding   1.9 - 6.3 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.8 x 10^6 (cells/1m
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   87.0% Additional information   
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