JCRB0132.1 AH601(JTC27)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0132.1 | Cell Name | AH601(JTC27) |
|---|---|---|---|
| Profile | A transplantable rat hepatoma cell line derived from ascites. | Other Name | JTC27 |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | liver | |
| Case history | hepatoma, Hepatoma induced by feeding aminoazotoluene. | Metastasis | |
| Tissue Metastasized | Genetics | hepatoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | transplantable rat ascites tumor cell line. Rat established on 1952 and tumor was maintained by transplantation. Primary cell culture was established on 1971. Serum free cell line was established on 1972. |
| Classify | tumor | Established by | Takaoka,T. & Katsuta,H. |
| Registered by | Takaoka,T. | Regulation for Distribution | free |
| Comment | Year | 2000 | |
| Medium | DM201 medium with 2 % fetal bovine serum. Add fetal bovine serum at the concentration of 10% for initial cell attachment after thawing (one over night). | Methods for Passages | Cells harvested after treatment with 0.25% trypsin. |
| Cell Number on Passage | Split ratio = 1/8. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | ascites |
| Tissue Type | hepatoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
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| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:4340679 | Tissue culture of rat ascites hepatoma AH-601 cells. Huh N,Takaoka T,Katsuta H Jpn J Exp Med. 1972 Jun;42(3):249-62 |
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LOT Information
| Cell No. | JCRB0132.1 | Cell Name | AH601(JTC27) |
|---|---|---|---|
| LOT No. | 06232000 | Lot Specification | distribution |
| Medium | DM201 medium with 2% fetal calf serum (Intergen RB2405). | Temperature | 37 C |
| Cell Density at Seeding | 1-4 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin in 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2.3 x 10^6 | |
| Viability at cell freezing (%) | 99.3 | Antibiotics Used | free |
| Passage Number | P6* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | LDH, G6PD, NP. Rat. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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