JCRB0140 NCE16
Cell information
Important Notice(s)Supplements for the Serum free culture medium
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0140 | Cell Name | NCE16 |
|---|---|---|---|
| Profile | Normal ecto-cervical keratinocyte cells immortalized by HPV16 virus genome. Growth of this cell line is dependent to EGF. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | |
| Identity | available | Tissue for Primary Cancer | uterine cervix |
| Case history | normal keratinocyte immortalized with HPV16. | Metastasis | |
| Tissue Metastasized | Genetics | E6/E7 expressed. Telomerase expression observed. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | keratinocyte | Character | Normal ecto-cervical keratinocyte cells immortalized by HPV16. Growth is dependent to EGF. |
| Classify | immortalized | Established by | Yasumoto,S. |
| Registered by | Yasumoto,S. | Regulation for Distribution | Not defined. |
| Comment | Any comments for subculture methods. | Year | 1999 |
| Medium | MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. MCDB153 with < 0.5% FCS was also used in original report. | Methods for Passages | Cells are harvested after treatment with 0.1% trypsin and 0.01% EDTA. |
| Cell Number on Passage | Not defined. | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | uterine ectocervix |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:12821936 | Neurotrophin receptor p75(NTR) characterizes human esophageal keratinocyte stem cells in vitro. Okumura T,Shimada Y,Imamura M,Yasumoto S Oncogene. 2003 Jun 26;22(26):4017-26 |
| Pubmed id:9310137 | Two distinct human uterine cervical epithelial cell lines established after transfection with human papillomavirus 16 DNA. Ohta Y,Tsutsumi K,Kikuchi K,Yasumoto S Jpn J Cancer Res. 1997 Jul;88(7):644-51 |
| Pubmed id:9149908 | Time correlation of commitment to calcium-induced apoptosis and terminal differentiation in human ectocervical keratinocytes in suspension cultures. Kikuchi K,Tsutsumi K,Ohta Y,Yasumoto S Cell Growth Differ. 1997 May;8(5):571-9 |
| Pubmed id:8710384 | Telomerase activity in normal human epithelial cells. Yasumoto S,Kunimura C,Kikuchi K,Tahara H,Ohji H,Yamamoto H,Ide T,Utakoji T Oncogene. 1996 Jul 18;13(2):433-9 |
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LOT Information
| Cell No. | JCRB0140 | Cell Name | |
|---|---|---|---|
| LOT No. | 07242006 | Lot Specification | distribution |
| Medium | MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 10 ng EGF, and 50 ug/ml BPE | Temperature | 37 C |
| Cell Density at Seeding | 3 - 5 x 10^4 cells/ml | Methods for Passages | Cells were harvested after treatment with 0.1% crystalline trypsin and 0.02% EDTA. Enzyme treatment was terminated by equal volume of 0.1% soybean trypsin inhibitor in PBS. Medium change every 2-3days |
| Doubling Time | approx. 3-5 days | Cell Number in Vial (cells/1ml) | 6.0 x 10^5 |
| Viability at cell freezing (%) | 75.1 | Antibiotics Used | free |
| Passage Number | P27 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:11,13 D7S820:11,12 D16S539:11 VWA:16 TH01:7,9.3 AM:X TPOX:11 CSF1PO:11,13 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 0.1% methylcellulose - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0140 | Cell Name | NCE16 |
|---|---|---|---|
| LOT No. | 113099 | Lot Specification | distribution |
| Medium | MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 50 ug/ml bovine pituitary extract. | Temperature | 37 C |
| Cell Density at Seeding | 1-1.7 x 10^2 cells/sq.cm. | Methods for Passages | Cells harvested with 0.1% trypsin and 0.01% EDTA. Change medium every 2-3 days and subculture every 6-7 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
| Viability at cell freezing (%) | 93.3 | Antibiotics Used | free |
| Passage Number | P26 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP and LDH examined. human non-HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:11,13 D7S820:11,12 D16S539:11 VWA:16 TH01:7,10 AM:X TPOX:11 CSF1PO:11,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | FM-1 and culture medium (1/1 mix) with 10% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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