JCRB0151 T-Ag-MOSE
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0151 | Cell Name | T-Ag-MOSE |
|---|---|---|---|
| Profile | Cells are derived from normal ovary of adult C3H/He mouse and immortalized by the plasmid pEF321-T containing T antigen gene. Cells are tumorigenic in nude mice. | Other Name | |
| Animal | mouse | Strain | C3H/He |
| Genus | Mus | Species | musclus |
| Sex | F | Age | 8-weeks |
| Identity | Tissue for Primary Cancer | ovary | |
| Case history | normal ovary of adult C3H/He mouse | Metastasis | |
| Tissue Metastasized | Genetics | pEF321-T plasmid introduced cell line, laminin positive. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like, cobble-stone like. | Character | immortalized by T antigen containing plasmid pEF321-T, tumorigenic in nude mice |
| Classify | transformed | Established by | Kido,M. & Shibuya,M. |
| Registered by | Kido,M. | Regulation for Distribution | not applied |
| Comment | 5% CO2, 37 C culture. Original ampules were frozen with medium and 10% DMSO. Cells adheded less than 100%. Anchorage dependency is No. Original description. | Year | 1999 |
| Medium | Dulbecco's modified Eagle's medium 10% fetal bovine serum. | Methods for Passages | 0.25% trypsin treatment when cells are harvested. |
| Cell Number on Passage | 1.5 x 10^4 cells/sq.cm. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | ovary |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9820870 | Isolation and characterization of mouse ovarian surface epithelial cell lines. Kido M,Shibuya M Pathol Res Pract. 1998;194(10):725-30 |
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LOT Information
| Cell No. | JCRB0151 | Cell Name | T-Ag-MOSE |
|---|---|---|---|
| LOT No. | 07142000 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (Mitsubishi PFCS PVF001). | Temperature | 37 C |
| Cell Density at Seeding | 1.7 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Medium change every 1-2 days. Subculture every 4-5 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.5 x 10^6 |
| Viability at cell freezing (%) | 85.8 | Antibiotics Used | free |
| Passage Number | P7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, NP and LDH examined. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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