JCRB0153.2 tsJT16
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0153.2 | Cell Name | tsJT16 |
|---|---|---|---|
| Profile | Rat 3Y1 derived ts mutant cell line for cell cycle progression from G0 and G1 to S phase. | Other Name | tsJT16(3Y1) |
| Animal | rat | Strain | Fischer 344 |
| Genus | Rattus | Species | norvegicus |
| Sex | F | Age | fetus |
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | A ts mutant for cell cycle progression from G0 and G1 to S. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | MNNG-mutagenized rat 3Y1 derivative cell line with ts mutation. Cells grows at 34 C but is lethal at 40 C. |
| Classify | mutant | Established by | Ninomiya-Tsuji, J. and Ide,T. |
| Registered by | Kyoizumi,S. | Regulation for Distribution | free |
| Comment | Year | 2000 | |
| Medium | Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal calf serum. | Methods for Passages | Cells are harvested after treatment with 0.125% trypsin and 0.01% EDTA. Subculture once a week at split ratio=1/8 at 34 C. |
| Cell Number on Passage | 5-8 x 10^5 cells/60mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | embryo |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
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| Pubmed id:7850896 | tsJT16, a cell cycle ts mutant of rat fibroblast defective in early G0/G1 transition, fails to induce G1-cyclin and cdk2 genes after serum stimulation at the nonpermissive temperature. Ueohzono T,Tanida-Miyake E,Kato N,Ide T Cell Struct Funct. 1994 Oct;19(5):341-8 |
| Pubmed id:1782669 | A cell cycle ts mutant, tsJT16, is defective in p70 synthesis through protein kinase C-dependent and -independent pathways. Miyake E,Takasuka T,Sakayama M,Ide T Cell Struct Funct. 1991 Aug;16(4):315-21 |
| Pubmed id:2032311 | A nuclear labile protein, p70, is expressed exclusively during G0-S transition but not in G1 phase of a cell cycle ts mutant, tsJT16. Takasuka T,Sakayama M,Miyake E,Ishibashi S,Ide T Cell Struct Funct. 1991 Feb;16(1):81-5 |
| Pubmed id:2340588 | A temperature-sensitive cell-cycle mutant of mammalian cells, tsJT16, is defective in a function operating soon after growth stimulation. Takasuka T,Ninomiya-Tsuji J,Sakayama M,Ishibashi S,Ide T Cell Struct Funct. 1990 Feb;15(1):39-45 |
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LOT Information
| Cell No. | JCRB0153.2 | Cell Name | tsJT16 |
|---|---|---|---|
| LOT No. | 10112000 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB2405, Mitsubishi PFCS, PVF001). | Temperature | 34 C (ts) |
| Cell Density at Seeding | 5.7 x 10^3 cells/sq.cm. | Methods for Passages | Cells harvested after treartment with 0.125% trypsin and 0.01% EDTA. Change medium every 1-2 days and subculture every 3-4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 97.8 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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