JCRB0158.1 MY-M12
Cell information
Important Notice(s)Media for JCRB0157 TK, JCRB0158.0-0158.2 MY**, JCRB0159 SLVL
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0158.1 | Cell Name | MY-M12 |
|---|---|---|---|
| Profile | Subclone of the MY, human bone marrow mononuclear cells. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 52 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | patient diagnosed as having acute biphenotypic leukemia. See reference for details. | Metastasis | |
| Tissue Metastasized | Genetics | Chimeric BCR/ABL, BCR exon e1 and ABL exon a3. only t(2;3)(p16;q29) observed by FISH analysis. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Bone marrow mononuclear cells. |
| Classify | tumor | Established by | Inokuchi,K. et. al. |
| Registered by | Inokuchi,K. | Regulation for Distribution | not defined |
| Comment | Please refer NIHS0246.1 for the STR-PCR data in the Cell_ID section. | Year | 2000 |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | dilution |
| Cell Number on Passage | split | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9790503 | Establishment of a cell line with variant BCR/ABL breakpoint expressing P180BCR/ABL from late-appearing Philadelphia-positive acute biphenotypic leukemia. Inokuchi K,Shinohara T,Futaki M,Hanawa H,Tanosaki S,Yamaguchi H,Nomura T,Dan K Genes Chromosomes Cancer. 1998 Nov;23(3):227-38 |
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LOT Information
| Cell No. | JCRB0158.1 | Cell Name | MY-M12 |
|---|---|---|---|
| LOT No. | 03202001 | Lot Specification | distribution |
| Medium | alpha-MEM medium with 10% fetal calf serum (Intergen RB24045). | Temperature | 37 C |
| Cell Density at Seeding | 1.1 x 10^5 cells/ml | Methods for Passages | Subculture cells by dilution. Suspension cell culture. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.8 x 10^6 |
| Viability at cell freezing (%) | 98.9 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(type B), NP, LDH. Human not HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:8 D7S820:10,13 D16S539:9 VWA:17,18 TH01:9 AM:X TPOX:8,11 CSF1PO:11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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