-Detailed Information [JCRB0161]-

--- On the small particles appear in cultures of Kasumi-series cell lines ---

(--- Kasumiシリーズ細胞株の培養に現れる微粒子について　---)

During the cultures of Kasumi-series cell lines, self propagating small particles, like microorganisms, appear in the culture medium. These particles were investigated by Iwate University (*1), and were reported that they are consisted mainly of hydroxy apatite and fetuin but are lacking nucleic acids (DNA, RNA). These revealed not to be mycoplasmal, bacteria or yeast conatmination.

(*1) Harasawa,R. et al., In vitro Cell. Dev. Biol. Anim. 42: 13-15, 2006.

At present, it is unknown why these particles increase, and there is no evidence that these are live particles.

Kasumiシリーズ細胞株の培養には微生物様の微細な顆粒が出現して増加します。この顆粒は、岩手大の解析により、リン酸カルシウムとフェチュインを主成分とし、核酸がみられないことが確認されており、マイコプラズマ、細菌、真菌（酵母）ではありません。この粒子がなぜ増えるのかは不明であり、生物であるという証拠はありません。

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[Cultivation of Kasumi-4]

The size of Kasumi-4 is very small, and when you collect the cells by centrifugation, please centrifuge the cells at 1500 rpm for 10 min.

When you start the culture, please seed the cells to T-25 flask with 2-3 mL of medium and the flask should be slanted (or to small well) so as to achieve the local high cell density. 

The growth of Kasumi-4 is very slow. The recommended split ratio is 1/2.

When you cultivate GM-CSF dependent cell lines, the GM-SCF should be newly added from its stock solution "in each time" of subcultivation or dilution.

If you passage the cells by dilution for suspension culture, please add the GM-CSF to the 10 ng/mL against to TOTAL VOLUME of medium (old medium + new medium).
This is because the GM-CSF may be consumpted by the cells in the old medium.

For example, if you dilute the cells by adding 10 mL new medium to 10 mL old medium, please add new GM-CSF to 10 ng/mL against 20 mL medium.

[Kasumi-4の培養]

Kasumi-4は細胞のサイズが大変小さく、細胞を遠心で回収する際には1500 rpm, 10分の遠心を推奨します。

培養開始時には、局所的な高細胞密度となるよう、細胞は2-3 mLの培地にサスペンドしてT-25フラスコで傾けて培養、または小さなウェルで培養を開始してください。

本細胞の増殖は極めて遅く、split ratioは1/2を推奨しています。

Kasumi-4はGM-CSF依存性の細胞です。GM-CSFはストック溶液から継代や希釈の都度添加するようにしてください。

希釈により継代する際には、GM-CSFは、培地の全容量（古い培地と新しい培地の総量）に対して10 ng/mLとなるように添加します。これは培養中の培地でGM-CSFが消費されている可能性があるためです。

たとえば10 mLの培養中の培地に10 mLの新しい培地を添加する際には、20 mLの培地量に対して新たに10 ng/mLとなるようGM-CSFを添加します。







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LOT Information

JCRB No.	JCRB0161	Cell Name	Kasumi-4
Profile	A novel human leukemia cell line established from a patient with chronic myelogenous leukaemia (CML) in blast crisis.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	6
Identity	available	Tissue for Primary Cancer	blood
Case history	chronic myelogenous leukaemia (CML) in blast crisis.	Metastasis
Tissue Metastasized		Genetics	t(9:22:11), overexpression of Evi-1 gene
Life Span	infinite	Crisis PDL
Morphology	myeloblast	Character	CD34+, CD33+, CD13+
Classify	tumor	Established by	Asou,H.
Registered by	Kyoizumi,S. & Asou,H	Regulation for Distribution	free
Comment		Year	2000
Medium	RPMI1640 medium with 10-20% heat inactivated fetal calf serum and 10ng/ml GM-CSF.	Methods for Passages	Subculture twice a week at split ratio of 1/2 by simple dilution. Suspension cell culture. The size of cells is small, and when you collect the cells, centrifuge at 1500 rpm for 10 min.
Cell Number on Passage	Split ratio = 1/2.	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	blood
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested	Detected RNA Virus	tested
CMV	-	parvoB19	-	HCV	-	HTLV-1	-
EBV	-	HBV	-	HIV-1	-	HTLV-2	-
HHV6	-	HTLV-1	-	HIV-2	-	HAV	-
HHV7	-	HTLV-2	-	-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
BKV	-	HIV-1	-
JCV	-	HIV-2	-
ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0613

Reference
Pubmed id:8611478	Establishment of a myeloid leukaemia cell line (Kasumi-4) with t(9;22;11)(q34;q11;q13), inv(3)(q21q26) and the EVI1 gene activation from a patient with chronic myelogenous leukaemia in blast crisis. Asou H,Eguchi M,Suzukawa K,Morishita K,Tanaka K,Date M,Hamamoto K,Kamada N Br J Haematol. 1996 Apr;93(1):68-74

Research results by users. Click！
Pubmed id:32180206	Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations. Kasai F,Asou H,Ozawa M,Kobayashi K,Kuramitsu H,Satoh M,Kohara A,Kaneko Y,Kawamura M Hum Cell. 2020 Jul;33(3):868-876

04282011
01122018

Cell No.	JCRB0161	Cell Name	Kasumi-4
LOT No.	04282011	Lot Specification	distribution
Medium	RPMI1640 medium with 10ng/ml GM-CSF and 20% heat inactivated fetal bovine serum.	Temperature	37 C
Cell Density at Seeding	1.5-5x10^5 cells/ml	Methods for Passages	Dilution (split ratio=1/2)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.89x10^6
Viability at cell freezing (%)	83.6	Antibiotics Used
Passage Number	p8*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11,12 D13S317:10,12 D7S820:8,10 D16S539:9,13 VWA:16,18 TH01:8,9 AM:X TPOX:8,11 CSF1PO:10,12
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0161	Cell Name	Kasumi-4
LOT No.	01122018	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) and 10 ng/ml GM-CSF.	Temperature	37 C
Cell Density at Seeding	1.4-1.9x10^5 cells/ml	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.0x10^6
Viability at cell freezing (%)	89.0	Antibiotics Used	free
Passage Number	p9*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11,12 D13S317:10,12 D7S820:8,10 D16S539:9,13 VWA:16,18 TH01:8,9 AM:X TPOX:8,11 CSF1PO:10,12
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	NT	Additional information	Cell viability of this cell line is relatively low after freeze-thaw. For successful reestablishment of the cell culture, collect cells from two vials into one tube in a total of 10ml medium. Then, centrifuge cells at higher speed, 1500 rpm for 10 min, than usual. Resuspend the cells in fresh 2ml medium and start cell culture at high cell density using either one 6 cm culture dish or one 25 cm culture flask.

Images

JCRB Cell Bank

JCRB0161 Kasumi-4

Cell information

LOT Information