JCRB0172 RMG-I
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0172 | Cell Name | RMG-I |
|---|---|---|---|
| Profile | Human tumor cell line from ovarian mesonephroid adenocarcinoma. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 34 |
| Identity | available | Tissue for Primary Cancer | ovary |
| Case history | ovarian mesonephroid adenocarcinoma | Metastasis | |
| Tissue Metastasized | Genetics | tumor markers = CA-125, TPA, BFP, Behta-micro-globulin. Mode of chrosomosome = 47. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | not informed | Character | Doubling time = 60 hrs. Colonyformation rate = 81 %. |
| Classify | tumor | Established by | Nozawa,S. et.al. |
| Registered by | Nozawa,S. | Regulation for Distribution | |
| Comment | Cell culture initiated by cell dispersion culture method from suspension cells in ascites and cloned later. Passage is over 104 when deposited to the FDSC. Cells were uniqure confirmed by the STR-PCR method. | Year | 1990 |
| Medium | Ham's F12 medium 90% with 10% fetal bovine serum. | Methods for Passages | Cells are subcultured after treatment with 0.02% EDTA and 2.5 % trypsin. |
| Cell Number on Passage | 7 x 10^4 cells/sq.cm. | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | ovary |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:3154025 | Establishment of a human ovarian clear cell carcinoma cell line (RMG-I) and its single cell cloning--with special reference to the stem cell of the tumor. Nozawa S,Tsukazaki K,Sakayori M,Jeng CH,Iizuka R Hum Cell. 1988 Dec;1(4):426-35 |
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LOT Information
| Cell No. | JCRB0172 | Cell Name | |
|---|---|---|---|
| LOT No. | 07052011 | Lot Specification | distribution |
| Medium | Ham's F12 medim with 10% fetal bovine serum (FBS: GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 30 hrs. | Cell Number in Vial (cells/1ml) | 2.7 x 10^6 |
| Viability at cell freezing (%) | 72.1 | Antibiotics Used | free |
| Passage Number | Unknown (15 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0172 | Cell Name | RMG-1 |
|---|---|---|---|
| LOT No. | 06212001 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal bovine serum (Intergen RB52405). | Temperature | 37 C |
| Cell Density at Seeding | 2.2 x 10^5 cells/ml | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 5 minutes. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
| Viability at cell freezing (%) | 92.7 | Antibiotics Used | free |
| Passage Number | P12* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0172 | Cell Name | RMG-I |
|---|---|---|---|
| LOT No. | 07142017 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). | Temperature | 37 C |
| Cell Density at Seeding | 0.7 - 1.4 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 31 hrs. | Cell Number in Vial (cells/1ml) | 3.4 x 10^6 |
| Viability at cell freezing (%) | 88.0 | Antibiotics Used | free |
| Passage Number | Unknown (15 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 88.0 | Additional information |
| Cell No. | JCRB0172 | Cell Name | RMG-I |
|---|---|---|---|
| LOT No. | 02042020 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1.1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 33 hrs. | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | Unknown (15 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:8,12 D7S820:11 D16S539:9,10 VWA:17,18 TH01:6,7 AM:X TPOX:11 CSF1PO:10 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90 | Additional information |
| Cell No. | JCRB0172 | Cell Name | RMG-I |
|---|---|---|---|
| LOT No. | 06132024 | Lot Specification | distribution |
| Medium | Ham's F-12 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 7.2 x 10^5 | |
| Viability at cell freezing (%) | 97.5 | Antibiotics Used | free |
| Passage Number | Unknown (15 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Ham's F-12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 91.1 | Additional information |