-Detailed Information [JCRB0181]-

JCRB0181 KP-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0181	Cell Name	KP-2
Profile	Human pancreatic tumor cell line.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	65
Identity	available	Tissue for Primary Cancer	pancreas
Case history	moderately differentiated tubular adenocarcinoma of ductal origin	Metastasis
Tissue Metastasized		Genetics	Mode of chromosome No.=142 at passage 20. CEA, CA, SLX were positive(passage27).
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	The tumor cell line is derived from pancreatic tissue originally. Adeno carcinoma cell line. The cells can be transplantable to nude mouse.
Classify	tumor	Established by	Ikeda,Y. et.al.
Registered by	Iguchi,H.	Regulation for Distribution	free
Comment	Cells are observed to be unique from the STR-PCR analysis.	Year	2001
Medium	RPMI1640 with 10% fetal calf serum.	Methods for Passages	0.25% trypsin and 0.1 % EDTA in PBS.
Cell Number on Passage	3 x 10^4 cells/60 mm dish	Race	Japanese
CO2 Conc.		Tissue Sampling	pancreas
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3004
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1298218

Reference
Pubmed id:2172194	Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice. Ikeda Y,Ezaki M,Hayashi I,Yasuda D,Nakayama K,Kono A Jpn J Cancer Res. 1990 Oct;81(10):987-93

Research results by users. Click！
Pubmed id:none	Cellular Membrane Localization of Innate Immune Checkpoint Molecule CD47 Is Regulated by Radixin in Human Pancreatic Ductal Adenocarcinoma Cells Takuro Kobori, Yui Ito, Yuka Sawada, Yoko Urashima, Takuya Ito and Tokio Obata Biomedicines.2023 Apr; 11(4): 1117

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LOT Information

09262008
04242003
07282014
05292018
04122021
11092021

Cell No.	JCRB0181	Cell Name
LOT No.	09262008	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	3 - 4 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Passage once a week.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	93.2	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0181	Cell Name
LOT No.	04242003	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F)	Temperature	37 C
Cell Density at Seeding	5-8 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T. =ca. 2.6 days	Cell Number in Vial (cells/1ml)	1.0 x 10^6
Viability at cell freezing (%)	91.1	Antibiotics Used	free
Passage Number	Unknown(6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS-RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0181	Cell Name	KP-2
LOT No.	07282014	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding		Methods for Passages	0.25% trypsin and 0.1 % EDTA in PBS.
Doubling Time	approx 2 days	Cell Number in Vial (cells/1ml)	7.4 x 10^5
Viability at cell freezing (%)	99	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	99	Additional information

Cell No.	JCRB0181	Cell Name	KP-2
LOT No.	05292018	Lot Specification	distribution
Medium	RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.7 - 1.5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 27 hrs.	Cell Number in Vial (cells/1ml)	2.6 x 10^6
Viability at cell freezing (%)	88.0	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI-1640	CO2 Conc.	5%
Viability immediately after thawing (%)	88.0	Additional information

Cell No.	JCRB0181	Cell Name	KP-2
LOT No.	04122021	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO:catalog No.11875) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820)	Temperature	37 C
Cell Density at Seeding	1.22-1.25x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.1% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.33x10^6
Viability at cell freezing (%)	100	Antibiotics Used	free
Passage Number	p8*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:9,13 D13S317:8,13 D7S820:10,12 D16S539:9,10 VWA:14 TH01:9 AM:X TPOX:8,11 CSF1PO:10,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

Images

Cell No.	JCRB0181	Cell Name	KP-2
LOT No.	11092021	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500, Lot S09336S1820)	Temperature	37 C
Cell Density at Seeding	0.3 - 1.1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 34 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	100	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	98.8	Additional information