JCRB0181 KP-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0181 Cell Name   KP-2
Profile   Human pancreatic tumor cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   65
Identity   available Tissue for Primary Cancer   pancreas
Case history   moderately differentiated tubular adenocarcinoma of ductal origin Metastasis   
Tissue Metastasized    Genetics   Mode of chromosome No.=142 at passage 20. CEA, CA, SLX were positive(passage27).
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   The tumor cell line is derived from pancreatic tissue originally. Adeno carcinoma cell line. The cells can be transplantable to nude mouse.
Classify   tumor Established by   Ikeda,Y. et.al.
Registered by   Iguchi,H. Regulation for Distribution   free
Comment   Cells are observed to be unique from the STR-PCR analysis. Year   2001
Medium   RPMI1640 with 10% fetal calf serum. Methods for Passages   0.25% trypsin and 0.1 % EDTA in PBS.
Cell Number on Passage   3 x 10^4 cells/60 mm dish Race   Japanese
CO2 Conc.    Tissue Sampling   pancreas
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3004
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298218
Reference
Pubmed id:2172194Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice.
Ikeda Y,Ezaki M,Hayashi I,Yasuda D,Nakayama K,Kono A
Jpn J Cancer Res. 1990 Oct;81(10):987-93


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LOT Information

Cell No.   JCRB0181 Cell Name   
LOT No.   09262008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage once a week.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   93.2 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0181 Cell Name   
LOT No.   04242003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   5-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. =ca. 2.6 days Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   91.1 Antibiotics Used   free
Passage Number   Unknown(6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0181 Cell Name   KP-2
LOT No.   07282014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.25% trypsin and 0.1 % EDTA in PBS.
Doubling Time   approx 2 days Cell Number in Vial (cells/1ml)   7.4 x 10^5
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   
Cell No.   JCRB0181 Cell Name   KP-2
LOT No.   05292018 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.7 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   88.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI-1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88.0 Additional information   
Cell No.   JCRB0181 Cell Name   KP-2
LOT No.   04122021 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO:catalog No.11875) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   1.22-1.25x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.1% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.33x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:8,13
D7S820:10,12
D16S539:9,10
VWA:14
TH01:9
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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Cell No.   JCRB0181 Cell Name   KP-2
LOT No.   11092021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500, Lot S09336S1820) Temperature   37 C
Cell Density at Seeding   0.3 - 1.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   98.8 Additional information   
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