JCRB0182 KP-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0182 Cell Name   KP-4
Profile   Human tumor cell line secreting parathyroid hormone-related peptide (PTHrP) established from pancreatic tumor. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   50
Identity   available Tissue for Primary Cancer   pancreas
Case history   pancreatic ductal cell carcinoma Metastasis   Yes
Tissue Metastasized   ascites Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Tumor cell line secreting parathyroid hormone-related peptide (PTHrP). Transplantable to nude mice.
Classify   tumor Established by   Nishi,Y. et.al.
Registered by   Iguchi,H. et.al. Regulation for Distribution   non
Comment   Cells are observed to be unique from the STR-PCR analysis. Year   2001
Medium   Iscove's modified Dulbecco's medium with 20% fetal calf serum (original description). Current medium for propagation is RPMI 1640 medium with 10% fetal calf serum. Methods for Passages   Cells were prepared after treatment with 0.25% trypsin and 0.1% EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.    Tissue Sampling   pancreas
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1338
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 753572
Reference
Pubmed id:9613452Effect of retinoic acid on morphological changes of human pancreatic cancer cells on collagen gels: a possible association with the metastatic potentials.
Jimi S,Shono T,Tanaka M,Kono A,Yamada Y,Shudo K,Kuwano M
Oncol Res. 1998;10(1):7-14
Pubmed id:21559554Establishment and characterization of pthrp-producing human pancreatic-cancer cell-line.
Nishi Y,Haji M,Takayanagi R,Iguchi H,Shimazoe T,Hirata J,Nawata H
Int J Oncol. 1994 Jul;5(1):33-9

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LOT Information

Cell No.   JCRB0182 Cell Name   
LOT No.   12052011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   7 - 10 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   13 hrs ? (<24 hrs.) Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   94.8 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0182 Cell Name   
LOT No.   01262006 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.5 - 1.7 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.1% EDTA
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   Unknown (P8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0182 Cell Name   KP-4
LOT No.   01162018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal Bovine Serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   5.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
Cell No.   JCRB0182 Cell Name   KP-4
LOT No.   10262020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 44 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   97.4 Additional information   
Cell No.   JCRB0182 Cell Name   KP-4
LOT No.   07112023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   1.1 - 1.9 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 19 hrs. Cell Number in Vial (cells/1ml)   5.3 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:10
D7S820:10,11
D16S539:10
VWA:14
TH01:9,10
AM:X
TPOX:9,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
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