JCRB0192 OCUM-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0192 | Cell Name | OCUM-1 |
|---|---|---|---|
| Profile | Human gastric cancer cell line from poorly differentiated adenocarcinoma. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 38 |
| Identity | available | Tissue for Primary Cancer | stomach |
| Case history | gastric cancer, Borrmann type IV, poorly differentiated adenocarcinoma containing signet ring cells. | Metastasis | Yes |
| Tissue Metastasized | Genetics | karyotype=mode 50, near diploid | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like with clustering | Character | This cell line produces carcinoembryonic antigen(CEA), carbohydrate antigen 19-9 (CA19-9), cancer-associated antigen SPan-1, and expresses EGF receptor. Tumorigenic to nude mice (intramusclar transplantation). |
| Classify | tumor | Established by | Kubo,T. |
| Registered by | Hirakawa,K. | Regulation for Distribution | |
| Comment | Year | 2002 | |
| Medium | Dulbecco's modified Eagle's medium with 0.5mM sodium pyruvate and 10% fetal bovine serum. | Methods for Passages | Dilution (original description). Loosely attached cells were hravested by trypsinization (at cell bank). |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | stomach |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1922102 | Molecular characterization of monoclonal anti-steroid antibodies: primary structures of the variable regions of seven antibodies specific for 17 alpha-hydroxyprogesterone or 11-deoxycortisol and their pH-reactivity profiles. Sawada J,Mizusawa S,Terao T,Naito M,Kurosawa Y Mol Immunol. 1991 Oct;28(10):1063-72 |
| Pubmed id:1961183 | [Establishment and characterization of a new gastric cancer cell line (OCUM-1), derived from Borrmann type IV tumor]. Kubo T Nihon Geka Gakkai Zasshi. 1991 Oct;92(10):1451-60 |
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LOT Information
| Cell No. | JCRB0192 | Cell Name | |
|---|---|---|---|
| LOT No. | 11282012 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate and 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 1.3 - 2.7 x 10^5 cells/mL | Methods for Passages | Dilution (suspension culture) |
| Doubling Time | approx. 64 hrs | Cell Number in Vial (cells/1ml) | 2.2 x 10^6 |
| Viability at cell freezing (%) | 89.0 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0192 | Cell Name | |
|---|---|---|---|
| LOT No. | 09162009 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate and 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 2.1 - 3.4 x 10^5 cells/ml | Methods for Passages | Original description is dilution. However, most of cells attached loosely to substratum, and these cells were harvested with 0.25% trypsin. |
| Doubling Time | 49 hrs | Cell Number in Vial (cells/1ml) | 4.5 x 10^6 |
| Viability at cell freezing (%) | 96.9 | Antibiotics Used | free |
| Passage Number | Unknown (12 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0192 | Cell Name | |
|---|---|---|---|
| LOT No. | 12222004 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate (final 1.5 mM) and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 1 - 5 x 10^5 cells/ml | Methods for Passages | Original description is dilution. However, most of cells attached loosely to substratum, and these cells were harvested with 0.25% trypsin. |
| Doubling Time | approx. 38 hrs | Cell Number in Vial (cells/1ml) | 3.8 x 10^6 |
| Viability at cell freezing (%) | 99.1 | Antibiotics Used | free |
| Passage Number | Unknown (P11 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0192 | Cell Name | OCUM-1 |
|---|---|---|---|
| LOT No. | 08172016 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium (low glucose) with 0.5 mM sodium pyruvate and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). | Temperature | 37 C |
| Cell Density at Seeding | 0.7 - 2.4 x 10^5 cells/mL | Methods for Passages | Dilution (suspension culture). In some cases, loosely attached cells appear, and they can be hravested by trypsinization. |
| Doubling Time | approx. 26 hrs. | Cell Number in Vial (cells/1ml) | 4.7 x 10^6 |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | Unknown (11 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 92 | Additional information |
| Cell No. | JCRB0192 | Cell Name | OCUM-1 |
|---|---|---|---|
| LOT No. | 06252018 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). | Temperature | 37 C |
| Cell Density at Seeding | 1.8 - 3.7 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). In some cases, loosely attached cells appear, and they can be hravested by trypsinization. |
| Doubling Time | apprlx. 34 hrs. | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 |
| Viability at cell freezing (%) | 94 | Antibiotics Used | free |
| Passage Number | Unknown (10 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Y/N | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94 | Additional information |
| Cell No. | JCRB0192 | Cell Name | OCUM-1 |
|---|---|---|---|
| LOT No. | 02092023 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 0.5mM sodium pyruvate and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437). | Temperature | 37 C |
| Cell Density at Seeding | 1.0 - 5.5 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). In some cases, loosely attached cells appear, and they can be hravested by trypsinization. |
| Doubling Time | approx. 46 hrs. | Cell Number in Vial (cells/1ml) | 4.0 x 10^6 |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | Unknown (15 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Y/N | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 88 | Additional information |
| Cell No. | JCRB0192 | Cell Name | OCUM-1 |
|---|---|---|---|
| LOT No. | 11292024 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 0.5 mM sodium pyruvate (GIBCO:catalog No.11360-070) and 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K). | Temperature | 37 C |
| Cell Density at Seeding | 2.2-3.6x10^5 cells/ml | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.0x10^6 |
| Viability at cell freezing (%) | 83 | Antibiotics Used | free |
| Passage Number | p11* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,10 D13S317:10,11 D7S820:12 D16S539:11 VWA:14,17 TH01:9 AM:X TPOX:8,11 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 83.1 | Additional information |
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