JCRB0207 CaR-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0207 Cell Name   CaR-1
Profile   adenocarcinoma Other Name   CaR-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   rectum
Case history   adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   
Classify   tumor Established by   Kaneko,Y.
Registered by   Isaka,H. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle,s minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.26 % trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   rectum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1116
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 924108
Images



LOT Information

Cell No.   JCRB0207 Cell Name   CaR-1
LOT No.   01232012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5 - 15 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   91.2 Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   Mislabeled as JCRC0207. Correct no. is JCRB0207.
Cell No.   JCRB0207 Cell Name   CaR-1
LOT No.   08202010 Lot Specification   distribution
Medium   agle's minimum essential medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Split ratio=1/3-1/4(once a week)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8x10^6
Viability at cell freezing (%)   92.2 Antibiotics Used   free
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:9,12
D7S820:8,12
D16S539:9
VWA:14,18
TH01:6
AM:X
TPOX:8
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0207 Cell Name   CaR-1
LOT No.   08052010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Split ratio=1/3-1/4(once a week)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.40x10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:9,12
D7S820:8,12
D16S539:9
VWA:14,18
TH01:6
AM:X
TPOX:8
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0207 Cell Name   
LOT No.   090793 Lot Specification   distribution
Medium   MEM : 10% FCS Temperature   37
Cell Density at Seeding   2x10^4/ml Methods for Passages   0.25 % trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8.4 x 10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P2 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0207 Cell Name   
LOT No.   110598 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 55 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   80.0 Antibiotics Used   free
Passage Number   P9 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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