JCRB0215 HeLa TG Cap
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0215 | Cell Name | HeLa TG Cap |
|---|---|---|---|
| Profile | epitheloid carcinoma, 6-thioguanine, chloramphenicol-resistant | Other Name | HeLa TG Cap |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 31 |
| Identity | available | Tissue for Primary Cancer | uterine cervix |
| Case history | modified hela cells | Metastasis | |
| Tissue Metastasized | Genetics | cervix, TG-r, Cap-r | |
| Life Span | infinite | Crisis PDL | NT |
| Morphology | epithelial-like | Character | cervix, TG-r, Cap-r |
| Classify | Established by | Sekiguchi,T. | |
| Registered by | Isaka,H. | Regulation for Distribution | Report to Dr.Sekiguchi is required. |
| Comment | Resistant to thioguanine and chloramphenicol. | Year | 1985 |
| Medium | Eagle's minimal essential medium with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.25% trypsin |
| Cell Number on Passage | 2 x 10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | cervix |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | + | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9892199 | Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T Cancer Res. 1999 Jan 1;59(1):141-50 |
| Pubmed id:13271674 | The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure. EAGLE H J Exp Med. 1955 Nov 1;102(5):595-600 |
| Pubmed id:13052828 | Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. SCHERER WF,SYVERTON JT,GEY GO J Exp Med. 1953 May;97(5):695-710 |
| Images |
|---|
                     |
| Movies |
|---|
|
LOT Information
| Cell No. | JCRB0215 | Cell Name | |
|---|---|---|---|
| LOT No. | 10142005 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot, GIBCO 4218333S) | Temperature | 37 C |
| Cell Density at Seeding | 0.7 - 2.3 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 0.8 day | Cell Number in Vial (cells/1ml) | 6.2 x 10^6 |
| Viability at cell freezing (%) | 88.9 | Antibiotics Used | free |
| Passage Number | P7 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type A), AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0215 | Cell Name | HeLa TG Cap |
|---|---|---|---|
| LOT No. | 06162023 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum (SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 2.1x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.30x10^6 |
| Viability at cell freezing (%) | 96 | Antibiotics Used | free |
| Passage Number | p7 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:13.3 D7S820:8 D16S539:9,10 VWA:16,18 TH01:7 AM:X TPOX:12 CSF1PO:9,10 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94 | Additional information |
| Images |
|---|
|
|