JCRB0233.0 BALB-MC Cell information
Cell type:general cells
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JCRB No.
JCRB0233.0
Cell Name
BALB-MC
Profile
mammary cancer, (BALB/cHe mouse)
Other Name
BALB/c-MC
Animal
mouse
Strain
BALB/cHe
Genus
Mus
Species
musclus
Sex
Age
embryo
Identity
Tissue for Primary Cancer
mammary
Case history
Metastasis
Tissue Metastasized
Genetics
mammary cancer
Life Span
infinite
Crisis PDL
Morphology
Character
mammary cancer
Classify
Established by
Morimoto,J.
Registered by
Egashira,Y.
Regulation for Distribution
Comment
Year
1987
Medium
Eagle's minimal essential medium with 10% fetal calf serum
Methods for Passages
Cells are treated with 0.12-0.24 % trypsin and 0.01-0.02 % EDTA.
Cell Number on Passage
2x10^4 cells/ml
Race
CO2 Conc.
5 %
Tissue Sampling
mammary gland
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:3680103 Establishment and characterization of a new murine mammary tumor cell line, BALB/c-MC. Morimoto J,Imai S,Taniguchi Y,Tsubura Y,Hosick HL In Vitro Cell Dev Biol. 1987 Nov;23(11):755-8
Images
Movies
LOT Information
Cell No.
JCRB0233.0
Cell Name
LOT No.
051187
Lot Specification
distribution
Medium
MEM + 10 % FCS
Temperature
37
Cell Density at Seeding
1/4 - 1/5
Methods for Passages
0.12-0.24 % trypsin + 0.01-0.02 % EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
UNK
Viability at cell freezing (%)
NT
Antibiotics Used
penicilline/streptomycin
Passage Number
*P3
PDL
Sterility: MYCOPLASMA
+
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
LD, G6PD, NP, PEPB, AST, MD, MPI
Chromosome Mode
45
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0233.0
Cell Name
LOT No.
111488
Lot Specification
distribution
Medium
MEM + 10 % FCS
Temperature
37
Cell Density at Seeding
1/4 - 1/5
Methods for Passages
0.12-0.24 % trypsin + 0.01-0.02 % EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
1.0 x 10^6
Viability at cell freezing (%)
NT
Antibiotics Used
free
Passage Number
*P17
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
LD, G6PD, NP, PEPB, AST, MD, MPI
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0233.0
Cell Name
BALB-MC
LOT No.
02162015
Lot Specification
distribution
Medium
Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)
Temperature
37 C
Cell Density at Seeding
0.4 - 1.0 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 26 - 33 hrs
Cell Number in Vial (cells/1ml)
4.0 x 10^6
Viability at cell freezing (%)
65.2
Antibiotics Used
free
Passage Number
P26
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - EMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
65.2
Additional information