JCRB0248 ARLJ301-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0248 Cell Name   ARLJ301-3
Profile   liver epithelial cell, (F344/DuCrj rat) Other Name   ARLJ301-3
Animal   rat Strain   F344/DuCrj
Genus   Rattus Species   norvegicus
Sex   M Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   liver epithelial cell
Life Span    Crisis PDL   
Morphology    Character   liver epithelial cell
Classify    Established by   Furukawa,K.
Registered by   Furukawa, K. Regulation for Distribution   
Comment   10-week-old male F344/DuCrj strain rat. Year   1989
Medium   William's medium E with 10% fetal calf serum Methods for Passages   Cells are treated with 0.005% EDTA and 0.01% trypsin.
Cell Number on Passage   10^4 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4107
Reference
Pubmed id:3294781Enrichment and characterization of clonogenic epithelial cells from adult rat liver and initiation of epithelial cell strains.
Furukawa K,Shimada T,England P,Mochizuki Y,Williams GM
In Vitro Cell Dev Biol. 1987 May;23(5):339-48
Pubmed id:6616447Induction of transformation by six classes of chemical carcinogens in adult rat liver epithelial cells.
Shimada T,Furukawa K,Kreiser DM,Cawein A,Williams GM
Cancer Res. 1983 Nov;43(11):5087-92

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LOT Information

Cell No.   JCRB0248 Cell Name   ARLJ301-3
LOT No.   061195 Lot Specification   FDSC/distribution
Medium   William's E medium (Gibco,76K1547) with 10% fetal bovine serum (JRH12103-78P) Temperature   37 C
Cell Density at Seeding   4.9x10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.14x10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   P17* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0248 Cell Name   
LOT No.   061295 Lot Specification   distribution
Medium   William's E medium (Gibco,76K1547) with 10% fetal bovine serum (JRH12103-78P) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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