JCRB0263.1 PC12HS.P3
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0263.1 | Cell Name | PC12HS.P3 |
|---|---|---|---|
| Profile | Serum free NGF sensitive PC12HS cell line. | Other Name | PC12 clone HS.P3 |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | neural | |
| Case history | pheochromocytoma of the adrenal medulla. | Metastasis | No |
| Tissue Metastasized | Genetics | Serum free cell culture derived from NGF-highly sensitive clone (HS) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Cells are differentiated into sympathetic neuron-like extensions when they are exposed to NGF. |
| Classify | variant | Established by | Takaoka,T. |
| Registered by | Takaoka,T. | Regulation for Distribution | |
| Comment | Year | 2004 | |
| Medium | DM201. Serum free. | Methods for Passages | Cells were harvested with scraper. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | chromaffin cell |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1493541 | Different ability in PC12 clones to recover from MMC toxicity following NGF treatment. Iizuka H,Umeda M Brain Res. 1992 Dec 18;599(1):1-5 |
| Pubmed id:3839317 | Molecular cloning of a gene sequence regulated by nerve growth factor. Levi A,Eldridge JD,Paterson BM Science. 1985 Jul 26;229(4711):393-5 |
| Pubmed id:6641712 | A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12). Biocca S,Cattaneo A,Calissano P EMBO J. 1983;2(5):643-8 |
| Pubmed id:742018 | [Therapeutic results in adult acute leukemia in Giessen]. Löffler H,Pralle H,Graubner M,Schrezenmaier E Verh Dtsch Ges Inn Med. 1978;(84):156-8 |
| Pubmed id:1065897 | Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Greene LA,Tischler AS Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8 |
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LOT Information
| Cell No. | JCRB0263.1 | Cell Name | PC12HS.P3 |
|---|---|---|---|
| LOT No. | 08042004 | Lot Specification | distribution |
| Medium | DM201 medium. Serum free medium. | Temperature | 37 C |
| Cell Density at Seeding | 1-3 x 10^4 cells/sq.cm. | Methods for Passages | Cells were harvested with scraper. Subculture every 4-6 days and medium change every 2-4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 6.3 x 10^6 |
| Viability at cell freezing (%) | 98.5 | Antibiotics Used | MC210 to remove mycoplasmas. This culture only. |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 1 to 1 mixture of the culture medium with the FM-1 medium containing 10% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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