JCRB0268 PC12IS

Cell information

Important Notice(s)

PC12HS, MS, LSについて

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0268 Cell Name   PC12IS
Profile   NGF insensitive clone of rat PC12 cell line. Other Name   PC12 clone IS
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   pheochromocytoma of the adrenal medulla. Metastasis   
Tissue Metastasized    Genetics   NGF-insensitive clone (IS)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   This clone does not respond to NGF as determined by neurite outgrowth.
Classify   variant Established by   Iizuka,H. & Umeda,M.
Registered by   Umeda,M. Regulation for Distribution   
Comment    Year   1996
Medium   RPMI1640 medium with 10% heat inactivated horse serum and 5% fetal bovine serum. Methods for Passages   Cells are harvested by pipetting.
Cell Number on Passage   3-5 x 10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   chromaffin cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J440
Reference
Pubmed id:1493541Different ability in PC12 clones to recover from MMC toxicity following NGF treatment.
Iizuka H,Umeda M
Brain Res. 1992 Dec 18;599(1):1-5
Pubmed id:3839317Molecular cloning of a gene sequence regulated by nerve growth factor.
Levi A,Eldridge JD,Paterson BM
Science. 1985 Jul 26;229(4711):393-5
Pubmed id:6641712A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12).
Biocca S,Cattaneo A,Calissano P
EMBO J. 1983;2(5):643-8
Pubmed id:742018[Therapeutic results in adult acute leukemia in Giessen].
Löffler H,Pralle H,Graubner M,Schrezenmaier E
Verh Dtsch Ges Inn Med. 1978;(84):156-8
Pubmed id:1065897Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Greene LA,Tischler AS
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8

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LOT Information

Cell No.   JCRB0268 Cell Name   PC12IS
LOT No.   11242004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated horse serum and 5% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   3.5 x 10^4 cells /sq.cm Methods for Passages   Cells are harvested by pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.25x10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   NT
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, LDH examined. Rat.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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