-Detailed Information [JCRB0435]-

JCRB0435 JHH-4

Cell information

Important Notice(s)

On the agreement for distribution of JHH series cell lines, NOZ and OZ

JHH-series of Human hapatoma cell lines
OZ: Human bile duct carcinoma cell line
NOZ: Human gallbladder carcinoma cell line

The distribution of above cell lines is regulated by the depositor/developer, Dr. Nagamori, National Institute of Infectious Diseases in Japan.
Applicants should sign the extra agreement form (Form D) including the following conditions, in addition to the regular order form: Request and Agreement Form (Form A).

1. Adhere to publish the results obtained using the cell lines to scientific papers, etc. to the extent of disclosure.

2. Quote the establisher or references of the cell lines in publication using the cell lines.

3. Inform the publications obtained from the use of the cell lines, and send reprints to the establisher.

4. Do not use the cell line for the purpose other than described in Request and Agreement Form (Form A). If you need to use the cell lines for the other purpose, you are requested to send new Request and Agreement Form to cell bank.

Reprints should be sent to:

Prof. Seishi Nagamori
Department of Virology II
National Institute of Infectious Diseases
1-23-1 Toyama, Shinjyuku-ku, Tokyo 162-8640
Japan
E-mail: dr_jojo_39[atsign]icloud.com

[JHHシリーズ細胞株, NOZ, OZ細胞株について]

JHHシリーズ細胞株, NOZ, OZ細胞株につきましては、分譲依頼書・同意書に記載の事項に加え、寄託者・樹立者の永森靜志先生から、本細胞を用いて得られた成果は開示可能な範囲で論文・学会などで発表することを遵守し、別刷を永森先生宛に送付するよう指示されております。また、分譲依頼書・同意書に御記載の研究内容以外の研究目的でのご使用につきましては、再度同意書を頂く必要がございます。この内容につきまして、分譲のご依頼時に永森先生あての同意書をいただきます。どうかよろしくご了解ください。

永森先生連絡先

〒162-8640
東京都新宿区戸山1-23-1
国立感染症研究所
ウイルス第2部
永森 靜志
dr_jojo_39[atsign]icloud.com

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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0435	Cell Name	JHH-4
Profile	Human hepatocellular carcinoma cell line.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	51
Identity	available	Tissue for Primary Cancer	liver, gallbladder
Case history	hepatocellular carcinoma	Metastasis
Tissue Metastasized		Genetics	HBV integration is not observed.
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Alpha-phetoprotein and albumin-producing cell line. HBs antigen-negative. HCV was not detected by an RT-PCR method tested in the HSRRB.
Classify	tumor	Established by	Hasumura,S. et al.
Registered by	Nagamori,S.	Regulation for Distribution	Restricted: Cell's developer's approval is required
Comment	Cell Bank will handle to get the approval but the depositor may contact to the requested person directly.	Year	1988
Medium	Eagle's minimal essential medium with 10% fetal calf serum.	Methods for Passages	Cells are harvested after the treatment with 0.25% trypsin and 0.02% EDTA at room temp. for 5 min.
Cell Number on Passage	1-2x10^4 cells/sq.cm.	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	liver
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2787
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1240158

Reference
Pubmed id:2856444	[Establishment and characterization of a human hepatocellular carcinoma cell line JHH-4]. Hasumura S,Sujino H,Nagamori S,Kameda H Hum Cell. 1988 Mar;1(1):98-100

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Pubmed id:31324698	The 2-Hydroxyiminostilbene Metabolite of Carbamazepine or the Supernatant from Incubation of Hepatocytes with Carbamazepine Activates Inflammasomes: Implications for Carbamazepine-Induced Hypersensitivity Reactions. Kato R,Ijiri Y,Hayashi T,Uetrecht J Drug Metab Dispos. 2019 Oct;47(10):1093-1096
Pubmed id:29087505	Editor's Highlight: An Impaired Immune Tolerance Animal Model Distinguishes the Potential of Troglitazone/Pioglitazone and Tolcapone/Entacapone to Cause IDILI. Mak A,Kato R,Weston K,Hayes A,Uetrecht J Toxicol Sci. 2018 Feb 1;161(2):412-420
Pubmed id:28081667	Contributions of caspase-8 and -9 to liver injury from CYP2E1-produced metabolites of halogenated hydrocarbons. Ijiri Y,Kato R,Sadamatsu M,Takano M,Yasuda Y,Tanaka F,Oishi C,Imano H,Okada Y,Tanaka K,Hayashi T Xenobiotica. 2018 Jan;48(1):60-72
Pubmed id:28525267	Supernatant from Hepatocyte Cultures with Drugs That Cause Idiosyncratic Liver Injury Activates Macrophage Inflammasomes. Kato R,Uetrecht J Chem Res Toxicol. 2017 Jun 19;30(6):1327-1332
Pubmed id:27183710	Effect of hypoxia on UDP-glucuronosyl transferase mRNA expression in human hepatocarcinoma functional liver celL4 cell line. Kato R,Matsura A,Kamiya R,Oishi C,Kagawa Y,Tanaka F,Matsumoto N,Ijiri Y,Hayashi T Pharmazie. 2016 Mar;71(3):152-3
Pubmed id:24599999	Novel permissive cell lines for complete propagation of hepatitis C virus. Shiokawa M,Fukuhara T,Ono C,Yamamoto S,Okamoto T,Watanabe N,Wakita T,Matsuura Y J Virol. 2014 May;88(10):5578-94
Pubmed id:20044606	Infrequent amplification of JUN in hepatocellular carcinoma. Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T Anticancer Res. 2009 Dec;29(12):4989-94
Pubmed id:18248513	Chemosensitivity determinants of irinotecan hydrochloride in hepatocellular carcinoma cell lines. Takahata T,Ookawa K,Suto K,Tanaka M,Yano H,Nakashima O,Kojiro M,Tamura Y,Tateishi T,Sakata Y,Fukuda S Basic Clin Pharmacol Toxicol. 2008 Apr;102(4):399-407
Pubmed id:17549407	Activation of a system A amino acid transporter, ATA1/SLC38A1, in human hepatocellular carcinoma and preneoplastic liver tissues. Kondoh N,Imazeki N,Arai M,Hada A,Hatsuse K,Matsuo H,Matsubara O,Ohkura S,Yamamoto M Int J Oncol. 2007 Jul;31(1):81-7
Pubmed id:16041541	Interferon-alpha/beta upregulate IL-15 expression in vitro and in vivo: analysis in human hepatocellular carcinoma cell lines and in chronic hepatitis C patients during interferon-alpha/beta treatment. Yamaji K,Nabeshima S,Murata M,Chong Y,Furusyo N,Ikematsu H,Hayashi J Cancer Immunol Immunother. 2006 Apr;55(4):394-403
Pubmed id:16522372	A comparison of the antitumor effects of interferon-alpha and beta on human hepatocellular carcinoma cell lines. Murata M,Nabeshima S,Kikuchi K,Yamaji K,Furusyo N,Hayashi J Cytokine. 2006 Feb 7;33(3):121-8
Pubmed id:15349899	Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells. Yokoo H,Kondo T,Fujii K,Yamada T,Todo S,Hirohashi S Hepatology. 2004 Sep;40(3):609-17
Pubmed id:10766424	Involvement of insulin-like growth factor binding protein-3 in the retinoic acid receptor-alpha-mediated inhibition of hepatocellular carcinoma cell proliferation. Murakami K,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I Cancer Lett. 2000 Apr 3;151(1):63-70
Pubmed id:9446792	Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1. Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21
Pubmed id:8835345	Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines. Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M J Med Virol. 1996 Feb;48(2):133-40
Pubmed id:1315167	Induction of allogeneic tumour- and lymphokine-activated lymphocytes against hepatocellular carcinoma. Yasumura S,Higuchi K,Hioki O,Okada K,Tsukishiro T,Tsuchida T,Miyagiwa M,Nambu S,Yasuyama T,Inoue K J Gastroenterol Hepatol. 1992 Mar-Apr;7(2):136-41
Pubmed id:1651985	Preferential expression of the large hepatitis B virus surface antigen gene by an adenovirus-hepatitis B virus recombinant. Yuasa T,Kajino K,Saito I,Miyamura T J Gen Virol. 1991 Aug;72 ( Pt 8)():1927-34
Pubmed id:1701409	Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines. Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2543327	[Combination therapy of hyperthermia and other methods in liver and bile tract cancers--evaluation of these methods using cancer cell lines in vitro]. Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1905-12
Pubmed id:2856500	[Effects of TNF on human hepatocellular carcinoma cell lines and their modification by hyperthermia]. Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Satomi N Hum Cell. 1988 Jun;1(2):238-44
Pubmed id:2856444	[Establishment and characterization of a human hepatocellular carcinoma cell line JHH-4]. Hasumura S,Sujino H,Nagamori S,Kameda H Hum Cell. 1988 Mar;1(1):98-100
Pubmed id:3428672	An epidemic of pseudomembranous colitis: importance of person to person spread. Nolan NP,Kelly CP,Humphreys JF,Cooney C,O'Connor R,Walsh TN,Weir DG,O'Briain DS Gut. 1987 Nov;28(11):1467-73
Pubmed id:none	THE MORPHOLOGICAL STUDY OF HUMAN CULTURED HEPATOMA CELLS BY A NEW PLASMA POLYMERIZATION REPLICA METHOD AND TEM Hajime SUJINO Jikeikai Medical Journal 33(4), 379-400 (1986)
Pubmed id:none	ヒト肝癌細胞株に対するTumor necrosis factorの影響蓮村哲永森静志藤瀬清隆本間定筋野甫松浦知和清水恵一郎新谷稔里見信子医学のあゆみ 143(8), 655-656 (1987)
Pubmed id:none	Fluorochromeを用いたchromatin DNAのcolorimetric assay法による単層培養細胞増殖動態の検討松浦知和永森静志藤瀬清隆蓮村哲本間定筋野甫清水恵一郎新谷稔亀田治男医学のあゆみ 142(13), 957-958 (1987)
Pubmed id:none	樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム藤瀬清隆, 藤多和信, 永森静志, 蓮村哲, 本間定, 筋野甫, 松浦知和, 清水恵一郎, 新谷稔, 大野典也, 亀田治男肝臓 29(5), 697-698 (1988)

Images

Movies

Cell No.	JCRB0435	Cell Name
LOT No.	04142005	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	5 - 15 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA at room temperature for 5 min.
Doubling Time	approx. 40.5 hrs	Cell Number in Vial (cells/1ml)	2.3 x 10^6
Viability at cell freezing (%)	97.1	Antibiotics Used	free
Passage Number	P17	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Eagle's MEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0435	Cell Name	JHH-4
LOT No.	061897	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal calf serum (Mitsubishi PVF01).	Temperature	37 C
Cell Density at Seeding	1-2x10^6 cells/sq.cm.	Methods for Passages	Cells are harvested after the treatment with 0.25% trypsin and 0.02% EDTA at room temp. for 5 min.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.0x10^6
Viability at cell freezing (%)	92.0	Antibiotics Used	free
Passage Number	P13	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture mdium with 5% DMSO.	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0435	Cell Name	JHH-4
LOT No.	04142014	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal calf serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	4.7 - 9.0 x 10^4 cells/mL	Methods for Passages	Cells were harvested after the treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	96.0	Antibiotics Used	free
Passage Number	P17	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9,13 D13S317:12 D7S820:10,12 D16S539:12,13 VWA:14,17 TH01:6,9 AM:X TPOX:8,11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	96.0	Additional information	Cell Bank will handle to get the approval but the depositor may contact to the requester directly.

Cell No.	JCRB0435	Cell Name	JHH-4
LOT No.	01242022	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Biowest Cat. # S18200-500)	Temperature	37 C
Cell Density at Seeding	0.4 - 2.0 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	97	Antibiotics Used	free
Passage Number	P16	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	92	Additional information

JCRB Cell Bank

JCRB0435 JHH-4

Cell information

LOT Information