JCRB0528 TIG-103
Cell information
Important Notice(s)Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0528 | Cell Name | TIG-103 |
|---|---|---|---|
| Profile | skin, normal diploid fibroblast | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 69 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | skin,normal diploid fibroblast | |
| Life Span | Crisis PDL | ||
| Morphology | Character | skin,normal diploid fibroblast | |
| Classify | normal | Established by | Tky.Inst.Geront. |
| Registered by | Ooka,H. | Regulation for Distribution | |
| Comment | Year | 1989 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium. |
| Cell Number on Passage | 5-6x10^4 cells/cm^2 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
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LOT Information
| Cell No. | JCRB0528 | Cell Name | |
|---|---|---|---|
| LOT No. | 06262006 | Lot Specification | distribution |
| Medium | Eagle's minimam essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S)This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 3 - 5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS |
| Doubling Time | approx. 2.6 days | Cell Number in Vial (cells/1ml) | 8.2 x 10^5 |
| Viability at cell freezing (%) | 91.8 | Antibiotics Used | free |
| Passage Number | Unknown (4 at bank) | PDL | 19 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0528 | Cell Name | |
|---|---|---|---|
| LOT No. | R132 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% FBS. This stock was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 6-7 x 10^4 cells/cm2 | Methods for Passages | Wash in CMF-PBS.Add 0.25% trypsin. Incubate at 37Cfor 10-15 min. Suspend cells in medium |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2.2 x 10^6 | |
| Viability at cell freezing (%) | 77.4 | Antibiotics Used | |
| Passage Number | PDL | 18 | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0528 | Cell Name | TIG-103 |
|---|---|---|---|
| LOT No. | 02212014 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was forzen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 2.3 - 2.6 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 5.5 x 10^5 | |
| Viability at cell freezing (%) | 84.6 | Antibiotics Used | free |
| Passage Number | Unknown (7 at bank) | PDL | 24.4 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 84.6 | Additional information |