JCRB0533 TIG-112

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0533 Cell Name   TIG-112
Profile   skin, normal diploid fibroblast Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   40
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   No
Tissue Metastasized    Genetics   Normal diploid, 2N=46.
Life Span   finite Crisis PDL   PD51
Morphology   fibroblast-like Character   Normal skin fibroblast cells. PD at distribution, 15-20 PDL.
Classify   normal Established by   TIG
Registered by   TIG Regulation for Distribution   free
Comment    Year   1998
Medium   Eagle's basal medium with 10% fetal bovine serum. Methods for Passages   Subculture every 7 days. Split ratio, 1/4. 0.25 % tryplin, 10-15 min.
Cell Number on Passage   6-8x10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3197
Reference
Pubmed id:7556460Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.
Kondo H,Yonezawa Y
Exp Cell Res. 1995 Oct;220(2):501-4
Pubmed id:1614223Changes in the migratory ability of human lung and skin fibroblasts during in vitro aging and in vivo cellular senescence.
Kondo H,Yonezawa Y
Mech Ageing Dev. 1992 May;63(3):223-33

Images



LOT Information

Cell No.   JCRB0533 Cell Name   
LOT No.   08302010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2.6 - 2.8 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 58 hrs. Cell Number in Vial (cells/1ml)   8.4 x 10^5
Viability at cell freezing (%)   91.7 Antibiotics Used   free
Passage Number   Unknown (> P18) PDL   22.8
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0533 Cell Name   
LOT No.   PD15 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.25% trypsin. Sprit ratio 1:4
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   100 ug/ml streptmycin and 100 units/ml penicilline
Passage Number    PDL   15
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0533 Cell Name   
LOT No.   R122 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% FBS. This lot was frozen using DMSO. Temperature   37C
Cell Density at Seeding   split ratio 1:4 Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   100 ug/ml streptomycin and 100 units/ml penicillin
Passage Number    PDL   15
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
CLOSE