JCRB0615 NIH/3T3 clone 5611

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0615 Cell Name   NIH/3T3 clone 5611
Profile   A cell line which was established from NIH Swiss mouse embryo. Other Name   NIH/3T3
Animal   mouse Strain   Swiss NIH
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   whole embryo
Life Span    Crisis PDL   
Morphology    Character   Fibroblasts derived from whole embryo.
Classify    Established by   Aaronson,S.A.
Registered by   Yuasa,Y. Regulation for Distribution   
Comment    Year   
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Methods for Passages   Wash by PBS once then 0.1 % trypsin(PBS) and incubate at 37 C for 5 minutes.
Cell Number on Passage   0.5-1x10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_7220
Reference
Pubmed id:19340560Gene structures, biochemical characterization and distribution of rat melatonin receptors.
Ishii H,Tanaka N,Kobayashi M,Kato M,Sakuma Y
J Physiol Sci. 2009 Jan;59(1):37-47
Pubmed id:16234326Chemotaxis towards hyaluronan is dependent on CD44 expression and modulated by cell type variation in CD44-hyaluronan binding.
Tzircotis G,Thorne RF,Isacke CM
J Cell Sci. 2005 Nov 1;118(Pt 21):5119-28
Pubmed id:4087463Two NIH3T3 cell lines of different origins circulating in the world.
Iwamoto A,Masuda M,Yoshikura H
Jpn J Exp Med. 1985 Jun;55(3):129-31
Pubmed id:3918269Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells.
Mulcahy LS,Smith MR,Stacey DW
Nature. 1985 Jan 17-23;313(5999):241-3
Pubmed id:742018[Therapeutic results in adult acute leukemia in Giessen].
Löffler H,Pralle H,Graubner M,Schrezenmaier E
Verh Dtsch Ges Inn Med. 1978;(84):156-8
Pubmed id:4311790Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
Jainchill JL,Aaronson SA,Todaro GJ
J Virol. 1969 Nov;4(5):549-53

Images
Movies



LOT Information

Cell No.   JCRB0615 Cell Name   
LOT No.   06252007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   4-8 x 10^4 cells/ml (passage twice a week) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   1.5 days Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0615 Cell Name   
LOT No.   10172003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   0.5 x 10^3 cells/sq.cm Methods for Passages   0.1% trypsin in Ca, Mg-free PBS. Do not allow the culture to be completely confluent.
Doubling Time   D.T. = ca. 1.7 days Cell Number in Vial (cells/1ml)   3.8 x 10^5
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0615 Cell Name   
LOT No.   03132000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0615 Cell Name   NIH/3T3 clone 5611
LOT No.   11202013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   1.1 - 1.8 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Do not allow the culture to be completely confluent.
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97.3 Additional information   
Cell No.   JCRB0615 Cell Name   NIH/3T3 clone 5611
LOT No.   04262016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose type) with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the culture full confluency.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Cell No.   JCRB0615 Cell Name   NIH/3T3 clone 5611
LOT No.   10162017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% new born calf serum(biowest lot#:S14368S0750) Temperature   37 C
Cell Density at Seeding   0.503-3.0x10^4 cells/cm^2 Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.22x10^6
Viability at cell freezing (%)   98.93 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB0615 Cell Name   NIH/3T3 clone 5611
LOT No.   03082019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (NCS; Biowest Cat. # S0750) Temperature   37 C
Cell Density at Seeding   2.4 - 4.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% NCS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95.8 Additional information   
Cell No.   JCRB0615 Cell Name   NIH/3T3 clone 5611
LOT No.   12042020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% newborn calf serum. Temperature   37 C
Cell Density at Seeding   approx. 2.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   80 Additional information   
Cell No.   JCRB0615 Cell Name   NIH/3T3 clone 5611
LOT No.   02102022 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (NCS; Biowest Cat. # S0750) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% NCS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
CLOSE